RO8191 (Synonyms: CDM-3008; RO4948191)

Name: RO8191
Brand: MedChemExpress
SKU: HY-W063968
Rating: 5.0 (24 reviews)

Cat. No.: HY-W063968 Purity: 98.80%: Data Sheet
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RO8191 (CDM-3008), an imidazonaphthyridine compound, is an orally active and potent interferon (IFN) receptor agonist. RO8191 directly binds to IFNα/β receptor 2 (IFNAR2) and activates IFN-stimulated genes (ISGs) expression and JAK/STAT phosphorylation. RO8191 shows antiviral activity against both HCV and EMCV with an IC₅₀ of 200 nM for HCV replicon. RO8191 is a cccDNA modulator (CDM) through interferon-like activity and has anti-HBV activity.

For research use only. We do not sell to patients.

CAS No. : 691868-88-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 24 publication(s) in Google Scholar

24 Publications Citing Use of MCE RO8191

Bio/Physico-chemical Assay

Histological Imaging/Staining

Flow Cytometry

: RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660.; Protein expression and phosphorylation of CD4⁺ T cells treated with BSA, OA or OA+RO8191 (20 μM; 3 days) assessed by western blotting (n = 3 per group). The results showed that addition of RO8191 to OA-treated CD4⁺ T cells significantly reversed the down-regulation of pSTAT5A (Y694) as well as inhibition of IL-10 expression.

: RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660.; Representative images of p-STAT5A (Y694) in CD4⁺ T cells treated with OA or OA + RO8191 (20 μM; 3 days), with quantification of p-STAT5A (Y694). Immunofluorescence showed that the nucleus localization of pSTAT5A was enhanced after addition of RO8191 in OA-treated CD4⁺ T cells. scale bar, 20 μm.

: RO8191 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun:141:156660.; Flow cytometry analysis of the percentage of Th1, Th17 and Treg cells in CD4⁺ T cells treated with BSA, OA, or OA+. NS, non-significance. RO8191 (20 μM; 3 days) reversed the up-regulation of Th17 and suppression of Treg cell proportion, but not the promotion of Th1 cell frequency in OA-treated CD4⁺ T cells.

: RO8191 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Jul 28;10(1):341. [Abstract]; RO8191 (30 mg/kg; p.o.; once daily for 14 days). Representative haematoxylin and eosin (H&E) sections of tracheal allografts harvested on day 28 (left panel) and corresponding statistical diagram (right panel) showing the degree of stenosis.

: RO8191 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Jul 28;10(1):341. [Abstract]; RO8191 (30 mg/kg; p.o.; once daily for 14 days). Representative Masson staining sections of the transplanted trachea (left panel) and corresponding statistical charts (right panel) illustrating collagen deposition.

: RO8191 purchased from MedChemExpress. Usage Cited in: Cancer Immunol Res. 2023 May 3;11(5):583-599. [Abstract]; ISG-THP1 (WT) or ISG-THP1 (STING KO) cells were treated with cGAMP (0.5 μM), or RO8191 (2.5 μM) with or without various doses of podofilox for 24 h. Normalized values for ISRE luciferase activity induced by cGAMP (or RO8191) along with podofilox are shown in comparison to the activity triggered by DMSO. The results showed that podofilox only showed a tendency to enhance the ISRE-driven immune response when stimulated with RO8191, which is an agonist that directly binds to IFNAR2 and activates the expression of ISGs.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

RO8191 (CDM-3008), an imidazonaphthyridine compound, is an orally active and potent interferon (IFN) receptor agonist. RO8191 directly binds to IFNα/β receptor 2 (IFNAR2) and activates IFN-stimulated genes (ISGs) expression and JAK/STAT phosphorylation. RO8191 shows antiviral activity against both HCV and EMCV with an IC₅₀ of 200 nM for HCV replicon^[1]. RO8191 is a cccDNA modulator (CDM) through interferon-like activity and has anti-HBV activity^[2].

IC₅₀ & Target^[1]

JAK1

In Vitro

RO8191 (CDM-3008) exerts antiviral activity dependent on IFNAR2/JAK1, but is independent of IFNAR1/Tyk2. RO8191, an IFNAR2 agonist, stimulates IFN signals in mice^[1].
RO8191 (0.08, 0.4, 2, 10 μM; for 72 h) strongly suppresses HCV replicon activity at 72 h in a dose-dependent manner. RO8191 reduces levels of the proteins HCV NS3 and NS4A, which are localized mainly in the perinuclear region of the replicon cells^[1].
RO8191 (0.08-10 μM; for 72 h) results in the disappearance of viral proteins such as NS3, NS4A/B, and NS5A/B^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RO8191 (CDM-3008; 30 mg/kg; oral) significantly induces antiviral genes Oas1b, Mx1, and Pkr in the livers of six-week-old C57BL/6J mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

373.21

Formula

C₁₄H₅F₆N₅O

CAS No.

691868-88-9

Appearance

Solid

Color

Off-white to yellow

SMILES

FC(C1=C2C=CC3=NC(C4=NN=CO4)=CN3C2=NC(C(F)(F)F)=C1)(F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (13.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6795 mL	13.3973 mL	26.7946 mL
5 mM	0.5359 mL	2.6795 mL	5.3589 mL
10 mM	0.2679 mL	1.3397 mL	2.6795 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.5 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.34 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (279 KB) SDS (420 KB)

COA (253 KB)

Handling Instructions (2659 KB)

References

[1]. Hideyuki Konishi, et al. An Orally Available, Small-Molecule Interferon Inhibits Viral Replication. Sci Rep. 2012;2:259. [Content Brief]

[2]. Yutaka Furutani, et al. An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes. PLoS One. 2019 Jun 12;14(6):e0216139. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6795 mL	13.3973 mL	26.7946 mL	66.9864 mL
	5 mM	0.5359 mL	2.6795 mL	5.3589 mL	13.3973 mL
	10 mM	0.2679 mL	1.3397 mL	2.6795 mL	6.6986 mL

RO8191 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RO8191 (Synonyms: CDM-3008; RO4948191)

Customer Review

RO8191 Related Antibodies

24 Publications Citing Use of MCE RO8191

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All JAK Isoform Specific Products:

View All STAT Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

RO8191 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

RO8191 Related Classifications

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