2. Immunology/Inflammation
  3. Tim3 IFNAR
  4. TIM-3-IN-2

TIM-3-IN-2 is a TIM-3 inhibitor. TIM-3-IN-2 blocks the interactions of TIM-3 with PtdSer, CEACAM1 and Gal-9, and inhibits the immunosuppressive function of TIM-3. TIM-3-IN-2 restores IFNγ release from peripheral blood mononuclear cells. TIM-3-IN-2 inhibits the proliferation of acute myeloid leukemia cells. TIM-3-IN-2 can be used for the research of acute myeloid leukemia.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

TIM-3-IN-2

TIM-3-IN-2 Chemische Struktur

CAS. Nr. : 1113126-49-0

Größe Preis Verfügbarkeit Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 Auf Lager
Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
5 mg Auf Lager
10 mg Auf Lager
25 mg Auf Lager
50 mg Auf Lager
100 mg   Angebot einholen  
200 mg   Angebot einholen  

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

This product is a controlled substance and not for sale in your territory.

Kundenbewertung

Based on 1 publication(s) in Google Scholar

TIM-3-IN-2 Related Antibodies

Top Publications Citing Use of Products

  • Biologische Aktivität

  • Reinheit & Dokumentation

  • Verweise

  • Kundenbewertung

Beschreibung

TIM-3-IN-2 is a TIM-3 inhibitor. TIM-3-IN-2 blocks the interactions of TIM-3 with PtdSer, CEACAM1 and Gal-9, and inhibits the immunosuppressive function of TIM-3. TIM-3-IN-2 restores IFNγ release from peripheral blood mononuclear cells. TIM-3-IN-2 inhibits the proliferation of acute myeloid leukemia cells. TIM-3-IN-2 can be used for the research of acute myeloid leukemia[1].

In Vitro

TIM-3-IN-2 (A-41) binds to purified human TIM-3 protein, and SPR assays show that it has submicromolar affinity with a KD value of 0.61 μM[1].
TIM-3-IN-2 binds to purified human TIM-3 protein, and its affinity reaches the submicromolar level as detected by MST, with a KD of 0.58 μM[1].
TIM-3-IN-2 (200-1000 nM; 1 h) inhibits the interaction between immobilized human TIM-3 protein and CEACAM1 protein in a competitive ELISA assay[1].
TIM-3-IN-2 (200-1000 nM) inhibits the interaction between human TIM-3 protein and PtdSer on the surface of apoptotic Jurkat cells in a dose-dependent manner in flow cytometry assays[1].
TIM-3-IN-2 (1000 nM) inhibits the interaction between human TIM-3 protein and Gal-9 protein in a competitive ELISA assay[1].
TIM-3-IN-2 (5 μM) restores IFNγ production in human peripheral blood mononuclear cells (PBMCs) that is inhibited by recombinant TIM-3[1].
TIM-3-IN-2 (5 μM; 0-100 h) reduces the proliferative capacity of NucLight Red-labeled human monocytic AML cells THP-1 in a co-culture system with peripheral blood mononuclear cells (PBMCs), as measured by an Incucyte-based assay[1].
TIM-3-IN-2 (5 μM; 6 days) enhances the ability of human peripheral blood mononuclear cells (PBMC) to inhibit the proliferation of human acute myeloid leukemia cell line Kasumi-1 in co-culture assays[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TIM-3-IN-2 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: NucLight Red-labeled THP-1 human monocytic AML cells
Concentration: 5 μM
Incubation Time: 0, 20. 40, 60, 80, 100 h
Result: Decreased the proliferation of THP-1 cells in the PBMC coculture, with a red object count pattern similar to that of the anti-TIM-3 mAb positive control.

Cell Proliferation Assay[1]

Cell Line: Kasumi-1 human AML cells
Concentration: 5 μM
Incubation Time: 6 days (with 16 h thymidine labeling)
Result: Potentiated the ability of PBMCs to significantly inhibit the proliferation of Kasumi-1 cells.
Molekulargewicht

461.47

Formel

C25H23N3O6
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=C(C=C(C=C1)OC)OC)COC2=CC=CC(C3=NC(C4=CC=C(C=C4)OC)=NO3)=C2
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 50 mg/mL (108.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1670 mL 10.8349 mL 21.6699 mL
5 mM 0.4334 mL 2.1670 mL 4.3340 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 2.5 mg/mL (5.42 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1670 mL 10.8349 mL 21.6699 mL 54.1747 mL
5 mM 0.4334 mL 2.1670 mL 4.3340 mL 10.8349 mL
10 mM 0.2167 mL 1.0835 mL 2.1670 mL 5.4175 mL
15 mM 0.1445 mL 0.7223 mL 1.4447 mL 3.6116 mL
20 mM 0.1083 mL 0.5417 mL 1.0835 mL 2.7087 mL
25 mM 0.0867 mL 0.4334 mL 0.8668 mL 2.1670 mL
30 mM 0.0722 mL 0.3612 mL 0.7223 mL 1.8058 mL
40 mM 0.0542 mL 0.2709 mL 0.5417 mL 1.3544 mL
50 mM 0.0433 mL 0.2167 mL 0.4334 mL 1.0835 mL
60 mM 0.0361 mL 0.1806 mL 0.3612 mL 0.9029 mL
80 mM 0.0271 mL 0.1354 mL 0.2709 mL 0.6772 mL
100 mM 0.0217 mL 0.1083 mL 0.2167 mL 0.5417 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

TIM-3-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TIM-3-IN-21113126-49-0Tim3IFNART-Cell Immunoglobulin and Mucin Containing Protein-3CD366Interferon-α/β receptorInterferon-alpha/beta receptorTIM-3 inhibitorJurkat cellsKasumi-1 human AML cellsperipheral blood mononuclear cellsacute myeloid leukemia cellsTHP-1 human monocytic AML cellsacute myeloid leukemiaInhibitorinhibitorinhibit

Ihre kürzlich angesehenen Produkte:

Online-Anfrage

Your information is safe with us. * Required Fields.

Produktname

  

Requested Quantity *

Name des Antragstellers *

 

Anrede

E-Mail-Addresse *

 

Telefonnummer *

Department

 

Name der Organisation *

City

Bundesland

Country or Region *

      

Bemerkungen

Massenanfrage

Inquiry Information

Produktname:
TIM-3-IN-2
Art. -Nr.:
HY-155175
Menge:
MCE Japan Authorized Agent:

Kundenbewertung

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Produktname

  

Lot #

Name des Antragstellers *

 

E-Mail-Addresse *

Telefonnummer

 

Department *

Name der Organisation *

 

Country or Region *

Bemerkungen

SAFETY DATA SHEET (SDS)

English - EN (254 KB) Français - FR (254 KB) Deutsch - DE (254 KB) Norwegian - NO (254 KB) Español - ES (254 KB) Swedish - SV (254 KB) Italian - IT (254 KB) Korean - KR (254 KB) Portuguese - PT (254 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.