Tubulin polymerization-IN-74

Cat. No.: HY-W874392 Purity: 98.79%: Data Sheet
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Handling Instructions
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Tubulin polymerization-IN-74 (compound 11) is a Tubulin polymerization inhibitor, with IC₅₀ of 15 μM. Tubulin polymerization-IN-74 can be used in anticancer research.

For research use only. We do not sell to patients.

Tubulin polymerization-IN-74 Chemical Structure

CAS No. : 54491-43-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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10 g	Get quote

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Purity & Documentation
References
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Description

Tubulin polymerization-IN-74 (compound 11) is a Tubulin polymerization inhibitor, with IC₅₀ of 15 μM. Tubulin polymerization-IN-74 can be used in anticancer research^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	> 2500 nM Compound: 11	Growth inhibition of human MCF7 cells assessed as G2/M DNA content after 48 hrs Growth inhibition of human MCF7 cells assessed as G2/M DNA content after 48 hrs	[PMID: 15566282]

Molecular Weight

225.31

Formula

C₁₄H₁₁NS

CAS No.

54491-43-9

Appearance

Solid

Color

White to off-white

SMILES

C1(SC2=CNC3=C2C=CC=C3)=CC=CC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (221.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4383 mL	22.1916 mL	44.3833 mL
5 mM	0.8877 mL	4.4383 mL	8.8767 mL
10 mM	0.4438 mL	2.2192 mL	4.4383 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (22.19 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (256 KB) HNMR (247 KB) LCMS (113 KB)

Handling Instructions (2659 KB)

References

[1]. De Martino G, et al. Arylthioindoles, potent inhibitors of tubulin polymerization. J Med Chem. 2004 Dec 2;47(25):6120-3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4383 mL	22.1916 mL	44.3833 mL	110.9582 mL
	5 mM	0.8877 mL	4.4383 mL	8.8767 mL	22.1916 mL
	10 mM	0.4438 mL	2.2192 mL	4.4383 mL	11.0958 mL
	15 mM	0.2959 mL	1.4794 mL	2.9589 mL	7.3972 mL
	20 mM	0.2219 mL	1.1096 mL	2.2192 mL	5.5479 mL
	25 mM	0.1775 mL	0.8877 mL	1.7753 mL	4.4383 mL
	30 mM	0.1479 mL	0.7397 mL	1.4794 mL	3.6986 mL
	40 mM	0.1110 mL	0.5548 mL	1.1096 mL	2.7740 mL
	50 mM	0.0888 mL	0.4438 mL	0.8877 mL	2.2192 mL
	60 mM	0.0740 mL	0.3699 mL	0.7397 mL	1.8493 mL
	80 mM	0.0555 mL	0.2774 mL	0.5548 mL	1.3870 mL
	100 mM	0.0444 mL	0.2219 mL	0.4438 mL	1.1096 mL