Cat. No.: HY-N2468

Xylobiose

(Synonyms: 1,4-β-D-Xylobiose; 1,4-D-Xylobiose)

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Xylobiose (1,4-β-D-Xylobiose; 1,4-D-Xylobiose) is an orally active Claudin 2/CLDN2 inhibitor and HSP27 inducer. Xylobiose works by regulating intestinal barrier function and glucose and lipid metabolism-related signaling pathways. Xylobiose inhibits CLDN2 expression to reduce intestinal permeability, induces HSP27 to enhance cell protection, and regulates the miR-122a/miR-33a axis to inhibit liver lipid synthesis and improve insulin resistance. Xylobiose can strengthen intestinal barrier integrity, reduce blood sugar and blood lipid levels, and reduce oxidative stress and inflammatory response. Xylobiose can be used in the study of type 2 diabetes and metabolic syndrome.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 6860-47-5
Formula: C₁₀H₁₈O₉
Molecular Weight:282.24

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Xylobiose Chemical Structure

CAS No.: 6860-47-5

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Claudin-2

HSP27

All TNF Receptor Isoforms :

All HSP Isoforms :

In Vitro

Xylobiose (5-20 mg/mL; 3-24 h) down-regulates the gene and protein expression of Claudin 2 in human intestinal Caco-2 cells, up-regulates the gene and protein expression of heat shock proteins HSP27 and HSP70, and enhances the transepithelial electrical resistance (TER) of the cell monolayer, which may strengthen the intestinal barrier function by regulating signaling pathways such as PI3K^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

Human intestinal Caco-2 cells
Concentration:

5, 10, 20 mg/mL
Incubation Time:

3, 6, 12, 24 h
Result:

Downregulated CLDN2 expression, which is involved in paracellular permeability, and upregulated HSP27 and HSP70 expression, which are cytoprotective proteins. These changes indicated an improvement in the cell's functional state rather than a direct impact on cell viability.

In Vivo

Xylobiose (5% replacement of dietary sucrose; dietary intake; daily; 6 weeks) improves glucose tolerance in the db/db mouse diabetic model, reduces fasting blood glucose, HbA1c, insulin levels and HOMA-IR, reduces plasma total triglycerides, regulate liver miR-122a and miR-33a expression, inhibits liver lipid production-related gene expression, and reduces liver oxidative stress and inflammatory response^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:
Dosage:
Administration:
Result:

Animal Model: C57BL/KsJ-db/db male mice (17-24 g, 5-week-old), type 2 diabetes model induced by genetic factors^[2] - Dosage (solvent): 5% xylobiose replacing sucrose in the diet (dissolved in the diet) - Administration: Dietary intake, daily, for 6 weeks - Result: Did not affect the body weight change compared with the diabetic control group, while significantly reduced food and water intake.

Chemical Information

CAS No. 6860-47-5
Appearance Solid
Molecular Weight 282.24
Formula C₁₀H₁₈O₉
Color White to off-white
SMILES

O=C[C@H](O)[C@@H](O)[C@@H](CO)O[C@H]1[C@@H]([C@H]([C@H](O)CO1)O)O
Synonyms

1,4-β-D-Xylobiose; 1,4-D-Xylobiose
Structure Classification
- Saccharides
- Polysaccharides
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 125 mg/mL (442.89 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5431 mL

17.7154 mL

35.4308 mL

5 mM

0.7086 mL

3.5431 mL

7.0862 mL

10 mM

0.3543 mL

1.7715 mL

3.5431 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (354.31 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Rini DM, et al. Xylobiose treatment strengthens intestinal barrier function by regulating claudin 2 and heat shock protein 27 expression in human Caco-2 cells. J Sci Food Agric. 2024 Mar 15;104(4):2518-2525. [Content Brief]

[2]. Lim E, et al. Xylobiose, an Alternative Sweetener, Ameliorates Diabetes-Related Metabolic Changes by Regulating Hepatic Lipogenesis and miR-122a/33a in db/db Mice. Nutrients. 2016 Dec 5;8(12):791 [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	3.5431 mL	17.7154 mL	35.4308 mL	88.5771 mL
	5 mM	0.7086 mL	3.5431 mL	7.0862 mL	17.7154 mL
	10 mM	0.3543 mL	1.7715 mL	3.5431 mL	8.8577 mL
	15 mM	0.2362 mL	1.1810 mL	2.3621 mL	5.9051 mL
	20 mM	0.1772 mL	0.8858 mL	1.7715 mL	4.4289 mL
	25 mM	0.1417 mL	0.7086 mL	1.4172 mL	3.5431 mL
	30 mM	0.1181 mL	0.5905 mL	1.1810 mL	2.9526 mL
	40 mM	0.0886 mL	0.4429 mL	0.8858 mL	2.2144 mL
	50 mM	0.0709 mL	0.3543 mL	0.7086 mL	1.7715 mL
	60 mM	0.0591 mL	0.2953 mL	0.5905 mL	1.4763 mL
	80 mM	0.0443 mL	0.2214 mL	0.4429 mL	1.1072 mL
	100 mM	0.0354 mL	0.1772 mL	0.3543 mL	0.8858 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Xylobiose

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All TNF Receptor Isoforms

All HSP Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Xylobiose Related Classifications

HY-N2468 Related Classifications