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  3. Uniblue A sodium

Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM).

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Uniblue A sodium Chemical Structure

Uniblue A sodium Chemical Structure

CAS No. : 14541-90-3

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Description

Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9.
2. Add 10 μL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 μL protein solution.
3. The samples are heated at 100°C for 1 min to perform the staining.
4. Add 100 μL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8.
5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis.
6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature.
7. Add 20 μL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes.
8. the samples are subjected to SDS-PAGE.
Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below).
As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

506.48

Formula

C22H15N2NaO7S2

CAS No.
Emission (Em)

594

Excitation (Ex)

555

SMILES

O=S(C(C(N)=C1C2=O)=CC(NC3=CC=CC(S(=O)(C=C)=O)=C3)=C1C(C4=C2C=CC=C4)=O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Uniblue A sodium Related Classifications

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Product Name:
Uniblue A sodium
Cat. No.:
HY-D1544
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