W(Dab)L 

W(Dab)L is a cationic membrane-disrupting antimicrobial peptide with protease stability, salt tolerance, and no induction of drug resistance. W(Dab)L is effective against planktonic MRSA, MRSA biofilms, and Pseudomonas aeruginosa, and exhibits low cytotoxicity to mammalian cells. As a bactericide, W(Dab)L selectively interacts with negatively charged bacterial membranes, triggering membrane permeabilization, deformation, disintegration, intracellular substance leakage, and ultimately leading to cell lysis. W(Dab)L maintains its chemical integrity and antimicrobial activity upon exposure to proteases and human serum. W(Dab)L can be used in studies of methicillin-resistant Staphylococcus aureus infections^[1].

W(Dab)L (15-20 μM) inhibits planktonic MRSA growth with an MIC of 15 μM in salt-free conditions and 20 μM in the presence of physiological salts, displaying partial salt tolerance^[1].
W(Dab)L (15 μM; up to 90 min) rapidly eliminates planktonic MRSA at its 15 μM MIC, achieving complete bacterial kill within 90 min^[1].
W(Dab)L (15 μM) inhibits 95% of S. aureus (MRSA) biofilm growth at its MBIC of 15 μM^[1].
W(Dab)L (30 μM) eradicates ~80% of 2-day mature S. aureus MRSA biofilms at its MBEC of 30 μM^[1].
W(Dab)L (15-50 μM; 4 h) shows minimal hemolysis (<25%) of human RBCs at 50 μM and no hemolysis at its 15 μM MIC^[1].
W(Dab)L (2.5-160 μM) is noncytotoxic to HeLa, HEK 293, and HDF cells at biologically active concentrations, with high cell survival observed even at concentrations up to 160 μM^[1].
W(Dab)L (Sub-MIC concentrations; 30 days, 96 bacterial generations) does not induce resistance development in MRSA over 96 bacterial generations when exposed to sub-MIC concentrations^[1].
W(Dab)L (15 μM; 6 h) remains stable after 6 h of incubation with a trypsin-chymotrypsin-proteinase K cocktail and retains full antimicrobial activity against MRSA with an MIC of 15 μM^[1].
W(Dab)L (15 μM; 6 h) remains stable after 6 h of incubation in human serum and retains full antimicrobial activity against MRSA with an MIC of 15 μM^[1].
W(Dab)L selectively interacts with bacterial membrane mimic SDS micelles (showing an 18 nm tryptophan fluorescence blue shift) and does not interact with mammalian membrane mimic DPC micelles^[1].
W(Dab)L disrupts the S. aureus (MRSA) inner membrane, increasing PI fluorescence by 50% relative to Triton X-100 control, confirming membrane permeabilization^[1].
W(Dab)L (15-30 μM) causes membrane deformation, blistering, and lysis of planktonic MRSA cells at 1× MIC (15 μM) and 2× MIC (30 μM), as observed via FESEM and TEM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1071.36

C₅₅H₈₆N₁₄O₈

Trp-{(Rac)-Dab}-Trp-Leu-{(Rac)-Dab}-{(Rac)-Dab}-Leu-Leu-NH2

W-{(Rac)-Dab}-WL-{(Rac)-Dab}-{(Rac)-Dab}-LL-NH2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yadav M, et al.. Salt-Tolerant, Protease-Stable and Non-Resistance Developing Cationic AMPs for Combatting Planktonic MRSA and its Biofilms. ACS medicinal chemistry letters. 2025 Jun 12;16(6):1089-1097.