α-Glucosidase-IN-23
Based on 1 Customer Validation
α-Glucosidase-IN-23 is an orally active α-Glucosidase inhibitor. α-Glucosidase-IN-23 decreases blood glucose by a-glucosidase inhibition with an IC50 value of 4.48 μM. α-Glucosidase-IN-23 can be used for the research of diabetes.
For research use only. We do not sell to patients.
- Purity: 98.37%
- CAS No.: 161187-57-1
- Formula: C20H23NO6
- Molecular Weight:373.40
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
IC50: 4.48 μM (a-glucosidase)[1].
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BJ | GI50 |
15.18 μM
Compound: 8b
|
Cytotoxicity against human BJ cells assessed as cell growth inhibition
Cytotoxicity against human BJ cells assessed as cell growth inhibition
|
[PMID: 36584305] |
| HEK293 | GI50 |
19.65 μM
Compound: 8b
|
Cytotoxicity against HEK293 cells assessed as cell growth inhibition
Cytotoxicity against HEK293 cells assessed as cell growth inhibition
|
[PMID: 36584305] |
| MRC5 | GI50 |
61.8 μM
Compound: 8b
|
Cytotoxicity against human MRC5 cells assessed as cell growth inhibition
Cytotoxicity against human MRC5 cells assessed as cell growth inhibition
|
[PMID: 36584305] |
| THLE-2 | GI50 |
67.8 μM
Compound: 8b
|
Cytotoxicity against human THLE-2 cells assessed as cell growth inhibition
Cytotoxicity against human THLE-2 cells assessed as cell growth inhibition
|
[PMID: 36584305] |
α-Glucosidase-IN-23 (compound 8b) (1 μM) shows prominent antidiabetic effect[1].
α-Glucosidase-IN-23 diminishes ROS in β-cells and muscle cells[1].
α-Glucosidase-IN-23 decreases blood glucose by a-glucosidase inhibition with an IC50 value of 4.48 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:INS-1 Cells
-
Concentration:1 μM
-
Incubation Time:
-
Result:Stimulated the growth of INS-1 cells in a dose-codependent approach.
-
Cell Line:INS-1 Cells
-
Concentration:1 μM
-
Incubation Time:
-
Result:Restored the viability of INS-1 cells by about five times from apoptosis and eight times from necrosis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Mice[1]
-
Dosage:0.2 mmol/kg
-
Administration:Oral; 0.2 mmol/kg/day
-
Result:
Cmax (nmol/mL) Tmax (h) T1/2(h) AUC (ng/h/mL) 10.11 ± 1.17 0.8 ± 0.0 1.90 ± 1.04 36.7 ± 2.23
Chemical Information
-
CAS No. 161187-57-1
-
Appearance Solid
-
Molecular Weight 373.40
-
Formula C20H23NO6
-
Color White to off-white
-
SMILES
CCOC(C1=C(NC(C)=C(C(OCC)=O)C1C2=C(OCO3)C3=CC=C2)C)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 28.57 mg/mL (76.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.86 mg/mL (7.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.86 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (28.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6781 mL | 13.3905 mL | 26.7809 mL | 66.9523 mL |
| 5 mM | 0.5356 mL | 2.6781 mL | 5.3562 mL | 13.3905 mL | |
| 10 mM | 0.2678 mL | 1.3390 mL | 2.6781 mL | 6.6952 mL | |
| 15 mM | 0.1785 mL | 0.8927 mL | 1.7854 mL | 4.4635 mL | |
| 20 mM | 0.1339 mL | 0.6695 mL | 1.3390 mL | 3.3476 mL | |
| 25 mM | 0.1071 mL | 0.5356 mL | 1.0712 mL | 2.6781 mL | |
| 30 mM | 0.0893 mL | 0.4463 mL | 0.8927 mL | 2.2317 mL | |
| 40 mM | 0.0670 mL | 0.3348 mL | 0.6695 mL | 1.6738 mL | |
| 50 mM | 0.0536 mL | 0.2678 mL | 0.5356 mL | 1.3390 mL | |
| 60 mM | 0.0446 mL | 0.2232 mL | 0.4463 mL | 1.1159 mL |
α-Glucosidase-IN-23 Related Classifications
HY-148575 Related Classifications
Powered by Bioz