2,4-Dihydroxypyrimidine-5-carboxylic Acid
Based on 1 Customer Validation
2,4-Dihydroxypyrimidine-5-carboxylic acid (Uracil-5-carboxylic acid) is a linker. 2,4-Dihydroxypyrimidine-5-carboxylic acid can be assembled with various metal ions. 2,4-Dihydroxypyrimidine-5-carboxylic acid can be enzymatically converted to Uracil (HY-I0960).
For research use only. We do not sell to patients.
- Purity : 99.90%
- CAS No.: 23945-44-0
- Formula: C5H4N2O4
- Molecular Weight:156.10
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
2,4-Dihydroxypyrimidine-5-carboxylic Acid (initial concentrations: 0.17-0.27 mM) is stoichiometrically converted to Uracil (HY-I0960) and Carbon dioxide by a partially purified enzyme preparation from Neurospora crassa[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 23945-44-0
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Appearance Solid
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Molecular Weight 156.10
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Formula C5H4N2O4
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Color White to light yellow
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SMILES
O=C(N1)NC=C(C(O)=O)C1=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (160.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (17.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.4061 mL | 32.0307 mL | 64.0615 mL | 160.1537 mL |
| 5 mM | 1.2812 mL | 6.4061 mL | 12.8123 mL | 32.0307 mL | |
| 10 mM | 0.6406 mL | 3.2031 mL | 6.4061 mL | 16.0154 mL | |
| 15 mM | 0.4271 mL | 2.1354 mL | 4.2708 mL | 10.6769 mL | |
| 20 mM | 0.3203 mL | 1.6015 mL | 3.2031 mL | 8.0077 mL | |
| 25 mM | 0.2562 mL | 1.2812 mL | 2.5625 mL | 6.4061 mL | |
| 30 mM | 0.2135 mL | 1.0677 mL | 2.1354 mL | 5.3385 mL | |
| 40 mM | 0.1602 mL | 0.8008 mL | 1.6015 mL | 4.0038 mL | |
| 50 mM | 0.1281 mL | 0.6406 mL | 1.2812 mL | 3.2031 mL | |
| 60 mM | 0.1068 mL | 0.5338 mL | 1.0677 mL | 2.6692 mL | |
| 80 mM | 0.0801 mL | 0.4004 mL | 0.8008 mL | 2.0019 mL | |
| 100 mM | 0.0641 mL | 0.3203 mL | 0.6406 mL | 1.6015 mL |
2,4-Dihydroxypyrimidine-5-carboxylic Acid Related Classifications
HY-66047 Related Classifications
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