5-Hydroxymethyluracil
Based on 1 Customer Validation
5-Hydroxymethyluracil is a product of oxidative DNA damage. 5-Hydroxymethyluracil can be used as a potential epigenetic mark enhancing or inhibiting transcription with bacterial RNA polymerase.
For research use only. We do not sell to patients.
- Purity : 99.85%
- CAS No.: 4433-40-3
- Formula: C5H6N2O3
- Molecular Weight:142.11
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-B0219
Allopurinol -
HY-I0960
Uracil -
HY-W004924R
5-Hydroxymethyluracil (Standard) -
HY-I0960R
Uracil (Standard) -
HY-W140956
3-Methyluracil -
HY-I0626
Cytosine -
HY-41340
2-Aminopyrimidine -
HY-I0626R
Cytosine (Standard) -
HY-60351
5-Aminopyrimidine -
HY-66047
2,4-Dihydroxypyrimidine-5-carboxylic Acid -
HY-69014
2-O-Methylcytosine -
HY-W002272
Isocytosine
All Endogenous Metabolite Isoforms
More
Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
Chemical Information
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CAS No. 4433-40-3
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Appearance Solid
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Molecular Weight 142.11
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Formula C5H6N2O3
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Color White to off-white
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SMILES
O=C1NC(C(CO)=CN1)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (351.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Djuric Z, et al. Quantitation of 5-(hydroxymethyl)uracil in DNA by gas chromatography with mass spectral detection. Chem Res Toxicol. 1991 Nov-Dec;4(6):687-91. [Content Brief]
[2]. Janoušková M, et al. 5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase. Chem Commun (Camb). 2017 Dec 12;53(99):13253-13255. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.0368 mL | 35.1840 mL | 70.3680 mL | 175.9201 mL |
| 5 mM | 1.4074 mL | 7.0368 mL | 14.0736 mL | 35.1840 mL | |
| 10 mM | 0.7037 mL | 3.5184 mL | 7.0368 mL | 17.5920 mL | |
| 15 mM | 0.4691 mL | 2.3456 mL | 4.6912 mL | 11.7280 mL | |
| 20 mM | 0.3518 mL | 1.7592 mL | 3.5184 mL | 8.7960 mL | |
| 25 mM | 0.2815 mL | 1.4074 mL | 2.8147 mL | 7.0368 mL | |
| 30 mM | 0.2346 mL | 1.1728 mL | 2.3456 mL | 5.8640 mL | |
| 40 mM | 0.1759 mL | 0.8796 mL | 1.7592 mL | 4.3980 mL | |
| 50 mM | 0.1407 mL | 0.7037 mL | 1.4074 mL | 3.5184 mL | |
| 60 mM | 0.1173 mL | 0.5864 mL | 1.1728 mL | 2.9320 mL | |
| 80 mM | 0.0880 mL | 0.4398 mL | 0.8796 mL | 2.1990 mL | |
| 100 mM | 0.0704 mL | 0.3518 mL | 0.7037 mL | 1.7592 mL |
5-Hydroxymethyluracil Related Classifications
HY-W004924 Related Classifications
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