5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

  • Chem Commun (Camb). 2017 Dec 12;53(99):13253-13255. doi: 10.1039/c7cc08053k.
Martina Janoušková  1 ,  Zuzana Vaníková ,  Fabrizia Nici ,  Soňa Boháčová ,  Dragana Vítovská ,  Hana Šanderová ,  Michal Hocek ,  Libor Krásný
Affiliations
  • 1. Dept. of Molecular Genetics of Bacteria, Institute of Microbiology of the Czech Academy of Sciences, CZ-14220 Prague 4, Czech Republic. [email protected].
PMID: 29184924 DOI: 10.1039/c7cc08053k
Abstract

DNA templates containing 5-hydroxymethyluracil or 5-hydroxymethylcytosine were used in an in vitro transcription assay with RNA polymerase from Escherichia coli. A strong enhancement of transcription was observed from DNA containing the Pveg promoter whereas a decrease was observed from DNA containing the rrnB P1 promoter, suggesting that they may act as epigenetic marks.

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