3-Acetylpyridine adenine dinucleotide, 90%
Based on 1 Customer Validation
3-Acetylpyridine adenine dinucleotide, 90% is an analog of nicotinamide adenine dinucleotide (NAD). 33-Acetylpyridine adenine dinucleotide, 90% collaboratively inhibits Lactate Dehydrogenase (LDH) with bisulfite.
For research use only. We do not sell to patients.
- Purity: 94.45%
- CAS No.: 86-08-8
- Formula: C22H28N6O14P2
- Molecular Weight:662.44
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 86-08-8
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Appearance Solid
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Molecular Weight 662.44
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Formula C22H28N6O14P2
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Color White to light yellow
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SMILES
O[C@H]1[C@](O[C@@H]([C@H]1O)COP(OP(OC[C@H]2O[C@]([N+]3=CC=CC(C(C)=O)=C3)([H])[C@@H]([C@@H]2O)O)([O-])=O)(O)=O)([H])N4C5=NC=NC(N)=C5N=C4
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Synonyms
3-APAD, 90%; 3-Acetylpyridine NAD, 90%
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 100 mg/mL (150.96 mM; Need ultrasonic)
DMSO : 50 mg/mL (75.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (7.55 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Boland MJ. NADH-dependent glutamate synthase from lupin nodules. Reactions with oxidised and reduced 3-acetylpyridine-adenine dinucleotide. Eur J Biochem. 1981 Apr;115(3):485-9. [Content Brief]
[2]. Ciaccio EI. The inhibition of lactate dehydrogenase by 3-acetylpyridine adenine dinucleotide and bisulfite. J Biol Chem. 1966 Apr 10;241(7):1581-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.5096 mL | 7.5479 mL | 15.0957 mL | 37.7393 mL |
| 5 mM | 0.3019 mL | 1.5096 mL | 3.0191 mL | 7.5479 mL | |
| 10 mM | 0.1510 mL | 0.7548 mL | 1.5096 mL | 3.7739 mL | |
| 15 mM | 0.1006 mL | 0.5032 mL | 1.0064 mL | 2.5160 mL | |
| 20 mM | 0.0755 mL | 0.3774 mL | 0.7548 mL | 1.8870 mL | |
| 25 mM | 0.0604 mL | 0.3019 mL | 0.6038 mL | 1.5096 mL | |
| 30 mM | 0.0503 mL | 0.2516 mL | 0.5032 mL | 1.2580 mL | |
| 40 mM | 0.0377 mL | 0.1887 mL | 0.3774 mL | 0.9435 mL | |
| 50 mM | 0.0302 mL | 0.1510 mL | 0.3019 mL | 0.7548 mL | |
| 60 mM | 0.0252 mL | 0.1258 mL | 0.2516 mL | 0.6290 mL | |
| H2O | 80 mM | 0.0189 mL | 0.0943 mL | 0.1887 mL | 0.4717 mL |
| 100 mM | 0.0151 mL | 0.0755 mL | 0.1510 mL | 0.3774 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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