1. Metabolic Enzyme/Protease
  2. Glycosidase
  3. 4-Aminophenyl-β-D-galactopyranoside, 98%

4-Aminophenyl-β-D-galactopyranoside, 98%  (Synonyms: 4-Aminophenyl-beta-D-galactopyranoside, 98%)

Cat. No.: HY-W011654 Pureza: 99.98%
Instrucciones de manejo Technical Support

4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer.

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4-Aminophenyl-β-D-galactopyranoside, 98%

4-Aminophenyl-β-D-galactopyranoside, 98% Estructura química

No. CAS : 5094-33-7

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
25 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtener un presupuesto  
500 mg   Obtener un presupuesto  

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Descripciòn

4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer[1][2][3].

In Vitro

4-Aminophenyl-β-D-galactopyranoside (0-1.7 mg/mL; 10 min-1 h) is used for cerium oxide nanoparticle-amplified colorimetric analysis, enabling sensitive detection of β-galactosidase activity with a limit of detection of 0.1 mU/mL and a linear range up to 2.0 mU/mL[1].
4-Aminophenyl-β-D-galactopyranoside (50-100 μM; 1-5 minutes) enables electrochemical detection of β-galactosidase derived from E. coli within 5 minutes under neutral pH and room temperature conditions; when incubated with substrate concentrations of 50 or 100 μM for 5 minutes, its limit of detection reaches 40 ng/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

271.27

Fòrmula

C12H17NO6

No. CAS
Appearance

Solid

Color

White to off-white

SMILES

OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=CC=C(C=C2)N

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 200 mg/mL (737.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6864 mL 18.4318 mL 36.8636 mL
5 mM 0.7373 mL 3.6864 mL 7.3727 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureza y Documentación

Purity: 99.98%

Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6864 mL 18.4318 mL 36.8636 mL 92.1591 mL
5 mM 0.7373 mL 3.6864 mL 7.3727 mL 18.4318 mL
10 mM 0.3686 mL 1.8432 mL 3.6864 mL 9.2159 mL
15 mM 0.2458 mL 1.2288 mL 2.4576 mL 6.1439 mL
20 mM 0.1843 mL 0.9216 mL 1.8432 mL 4.6080 mL
25 mM 0.1475 mL 0.7373 mL 1.4745 mL 3.6864 mL
30 mM 0.1229 mL 0.6144 mL 1.2288 mL 3.0720 mL
40 mM 0.0922 mL 0.4608 mL 0.9216 mL 2.3040 mL
50 mM 0.0737 mL 0.3686 mL 0.7373 mL 1.8432 mL
60 mM 0.0614 mL 0.3072 mL 0.6144 mL 1.5360 mL
80 mM 0.0461 mL 0.2304 mL 0.4608 mL 1.1520 mL
100 mM 0.0369 mL 0.1843 mL 0.3686 mL 0.9216 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
4-Aminophenyl-β-D-galactopyranoside, 98%
Cat. No.:
HY-W011654
Cantidad:
MCE Japan Authorized Agent: