4-Aminophenyl-β-D-galactopyranoside, 98%
Based on 1 Customer Validation
4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer.
For research use only. We do not sell to patients.
- Purity : 99.98%
- CAS No.: 5094-33-7
- Formula: C12H17NO6
- Molecular Weight:271.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
4-Aminophenyl-β-D-galactopyranoside (0-1.7 mg/mL; 10 min-1 h) is used for cerium oxide nanoparticle-amplified colorimetric analysis, enabling sensitive detection of β-galactosidase activity with a limit of detection of 0.1 mU/mL and a linear range up to 2.0 mU/mL[1].
4-Aminophenyl-β-D-galactopyranoside (50-100 μM; 1-5 minutes) enables electrochemical detection of β-galactosidase derived from E. coli within 5 minutes under neutral pH and room temperature conditions; when incubated with substrate concentrations of 50 or 100 μM for 5 minutes, its limit of detection reaches 40 ng/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5094-33-7
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Appearance Solid
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Molecular Weight 271.27
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Formula C12H17NO6
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Color White to off-white
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SMILES
OC[C@H]([C@@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=CC=C(C=C2)N
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Synonyms
4-Aminophenyl-beta-D-galactopyranoside, 98%
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (737.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (621 KB)
- English - EN (621 KB)
- Français - FR (621 KB)
- Deutsch - DE (621 KB)
- Norwegian - NO (621 KB)
- Español - ES (621 KB)
- Swedish - SV (621 KB)
- Italian - IT (621 KB)
- Korean - KR (621 KB)
- Portuguese - PT (621 KB)
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Handling Instructions (2659 KB)
References
[1]. Mustafa F, et al. Nanoparticle-based amplification for sensitive detection of β-galactosidase activity in fruits. Anal Chim Acta. 2021;1186:339129. [Content Brief]
[3]. Wang S, et al. Turn-on fluorometric and colorimetric dual-mode assay based on the in situ formation of silicon nanoparticles for the determination of β-galactosidase. Anal Chim Acta. 2025;1365:344260. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6864 mL | 18.4318 mL | 36.8636 mL | 92.1591 mL |
| 5 mM | 0.7373 mL | 3.6864 mL | 7.3727 mL | 18.4318 mL | |
| 10 mM | 0.3686 mL | 1.8432 mL | 3.6864 mL | 9.2159 mL | |
| 15 mM | 0.2458 mL | 1.2288 mL | 2.4576 mL | 6.1439 mL | |
| 20 mM | 0.1843 mL | 0.9216 mL | 1.8432 mL | 4.6080 mL | |
| 25 mM | 0.1475 mL | 0.7373 mL | 1.4745 mL | 3.6864 mL | |
| 30 mM | 0.1229 mL | 0.6144 mL | 1.2288 mL | 3.0720 mL | |
| 40 mM | 0.0922 mL | 0.4608 mL | 0.9216 mL | 2.3040 mL | |
| 50 mM | 0.0737 mL | 0.3686 mL | 0.7373 mL | 1.8432 mL | |
| 60 mM | 0.0614 mL | 0.3072 mL | 0.6144 mL | 1.5360 mL | |
| 80 mM | 0.0461 mL | 0.2304 mL | 0.4608 mL | 1.1520 mL | |
| 100 mM | 0.0369 mL | 0.1843 mL | 0.3686 mL | 0.9216 mL |
4-Aminophenyl-β-D-galactopyranoside, 98% Related Classifications
HY-W011654 Related Classifications
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