4′-O-Methylglabridin
Based on 1 Customer Validation
4′-O-Methylglabridin is an apoptosis inducer with antioxidant, cell cycle-disrupting and anticancer cytotoxic activities. 4′-O-Methylglabridin inhibits various cancer cell lines including liver cancer, breast cancer and colorectal cancer cell lines. By reducing the expression levels of phosphorylated Rb (Ser807/811) and p21 proteins, 4′-O-Methylglabridin promotes cell accumulation at the subG1 and G2/M phases, and triggers caspase-dependent apoptosis via cytochrome C release and caspase-9 activation. 4′-O-Methylglabridin also exerts antioxidant effects by inhibiting lipid peroxide levels and reducing β-carotene consumption, thereby blocking LDL oxidation. 4′-O-Methylglabridin can be used in the research of various cancers and atherosclerotic diseases.
For research use only. We do not sell to patients.
- Purity: 98.94%
- CAS No.: 68978-09-6
- Formula: C21H22O4
- Molecular Weight:338.40
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Caspase-9 |
4′-O-Methylglabridin (2.5-40 μM; 72 h) inhibits proliferation of Huh7, MCF7, and HCT116 cancer cell lines in vitro, with the strongest activity against Huh7 cells (IC50=9.6 μM)[1].
4′-O-Methylglabridin (IC50 concentration; 24 h, 48 h, 72 h) inhibits proliferation of Huh7 cells in a time-dependent manner, with IC50 values increasing from 7.31 μM at 24 h to 11.4 μM at 72 h[1].
4′-O-Methylglabridin (10 μM; 48 h) induces apoptotic nuclear changes in Huh7 cells after 48 h of treatment[1].
4′-O-Methylglabridin (10 μM; 48 h) induces caspase-dependent apoptosis in Huh7 cells after 48 h of treatment by increasing cytochrome C release and cleaved caspase-9 levels, while also reducing phospho-Rb, p53, Mdm2, and p21 levels to disrupt cell cycle regulation[1].
4′-O-Methylglabridin (50 μM; 90 min at 50°C) inhibits β-carotene consumption by 78% in a cell-free β-carotene-linoleate assay[2].
4′-O-Methylglabridin (30 μM; 2 h at 37°C) potently inhibits AAPH-induced oxidation of ex vivo human LDL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Huh7, MCF7, HCT116
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Concentration:2.5, 5, 10, 20, 40 μM
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Incubation Time:72 h
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Result:Inhibited proliferation of Huh7 cells with an IC50 of 9.6 μM.
Inhibited proliferation of MCF7 cells with an IC50 of 16.5 μM.
Inhibited proliferation of HCT116 cells with an IC50 of 10.8 μM.
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Cell Line:Huh7
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Concentration:IC50 concentration Test
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Incubation Time:24 h, 48 h, 72 h (monitored every 10 min for first 24 h, then every 30 min for remaining 48 h)
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Result:Inhibited Huh7 cell proliferation with an IC50 of 7.31 μM at 24 h.
Inhibited Huh7 cell proliferation with an IC50 of 9.8 μM at 48 h.
Inhibited Huh7 cell proliferation with an IC50 of 11.4 μM at 72 h.
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Cell Line:Huh7
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Concentration:10 μM
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Incubation Time:48 h
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Result:Increased the percentage of Huh7 cells in the subG1 phase to 5.26% relative to DMSO control.
Induced accumulation of Huh7 cells in the G2/M phase to 20.32% relative to DMSO control.
Induced changes in Huh7 cell nuclei consistent with apoptotic cell death, as detected by Hoechst staining.
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Cell Line:Huh7
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Concentration:10 μM (48 h IC50)
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Incubation Time:48 h
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Result:Increased cytochrome C and cleaved caspase-9 protein levels relative to DMSO control.
Decreased phospho-Rb (Ser807/811), p53, Mdm2, and p21 protein levels relative to DMSO control.
Did not increase cleaved PARP protein levels relative to DMSO control.
Chemical Information
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CAS No. 68978-09-6
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Appearance Solid
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Molecular Weight 338.40
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Formula C21H22O4
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Color White to off-white
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SMILES
CC1(OC2=C(C=C1)C3=C(C=C2)C[C@H](C4=C(C=C(C=C4)OC)O)CO3)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
4′-O-Methylglabridin Related Classifications
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