1. Apoptosis NF-κB
  2. Apoptosis NF-κB
  3. Astragalin

Astragalin  (Synonyms: Astragaline; 3-Glucosylkaempferol; Kaempferol 3-β-D-glucopyranoside)

Cat. No.: HY-N0015 Purity: 99.94%
Handling Instructions Technical Support

Astragalin (Astragaline) a flavonoid with anti-inflammatory, antioxidant, anticancer, bacteriostatic activity. Astragalin inhibits cancer cells proliferation and migration, induces apoptosis. Astragalin is orally active and provides nerve and heart protection, and resistance against and osteoporosis.

For research use only. We do not sell to patients.

CAS No. : 480-10-4

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10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Astragalin:

Top Publications Citing Use of Products

    Astragalin purchased from MedChemExpress. Usage Cited in: Bioresour Bioprocess. 2025 Dec 3;12(1):139.  [Abstract]

    CCK8 assay of Astragalin (1, 10, 20 μM) on AR42J cells.

    Astragalin purchased from MedChemExpress. Usage Cited in: Bioresour Bioprocess. 2025 Dec 3;12(1):139.  [Abstract]

    Live/dead staining showing living (green) and dying/dead (red) cells treated with Astragalin (10 μM).

    Astragalin purchased from MedChemExpress. Usage Cited in: Bioresour Bioprocess. 2025 Dec 3;12(1):139.  [Abstract]

    Apoptotic rate analyzed by Annexin V-FITC/PI staining and flow cytometry treated with Astragalin (10 μM).

    Astragalin purchased from MedChemExpress. Usage Cited in: Bioresour Bioprocess. 2025 Dec 3;12(1):139.  [Abstract]

    Images of H&E staining in pancreatic tissue treated with Astragalin (50, 75, 100 mg/kg, i.g.).

    Astragalin purchased from MedChemExpress. Usage Cited in: Bioresour Bioprocess. 2025 Dec 3;12(1):139.  [Abstract]

    Western blot of the NLRP3 signaling pathway treated with Astragalin (100 mg/kg, i.g.).

    View All NF-κB Isoform Specific Products:

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Astragalin (Astragaline) a flavonoid with anti-inflammatory, antioxidant, anticancer, bacteriostatic activity. Astragalin inhibits cancer cells proliferation and migration, induces apoptosis. Astragalin is orally active and provides nerve and heart protection, and resistance against and osteoporosis[1].

    IC50 & Target

    p65

     

    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    > 10 μM
    Compound: 6
    Cytotoxicity against human A549 cells after 48 hrs by SRB assay
    Cytotoxicity against human A549 cells after 48 hrs by SRB assay
    [PMID: 28165740]
    BT-549 IC50
    > 10 μM
    Compound: 6
    Cytotoxicity against human BT549 cells after 48 hrs by SRB assay
    Cytotoxicity against human BT549 cells after 48 hrs by SRB assay
    [PMID: 28165740]
    BV-2 IC50
    23.5 μM
    Compound: 6
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    [PMID: 28165740]
    BV-2 IC50
    77.36 μM
    Compound: 13
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    [PMID: 23149227]
    H9 EC50
    80 μg/mL
    Compound: 4
    Inhibitory activity against HIV-1 IIIB replication in H9 cell line
    Inhibitory activity against HIV-1 IIIB replication in H9 cell line
    10.1016/0960-894X(94)80015-4
    H9 IC50
    < 100 μg/mL
    Compound: 4
    Inhibitory activity against H9 cells uninfected with HIV-1
    Inhibitory activity against H9 cells uninfected with HIV-1
    10.1016/0960-894X(94)80015-4
    HCT-15 IC50
    23.5 μM
    Compound: 11
    Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay
    [PMID: 25981689]
    HL-60 IC50
    > 50 μg/mL
    Compound: 4
    Antioxidant activity in human HL60 cells assessed as reduction of cytochrome-c release
    Antioxidant activity in human HL60 cells assessed as reduction of cytochrome-c release
    [PMID: 10650074]
    Jurkat IC50
    > 100 μg/mL
    Compound: 9
    Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay
    Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay
    [PMID: 21546250]
    MDCK CC50
    143.3 μg/mL
    Compound: 8
    Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 15104496]
    MDCK CC50
    392.5 μg/mL
    Compound: 8
    Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 15104496]
    MDCK CC50
    > 300 μM
    Compound: 8
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    [PMID: 19729316]
    MDCK EC50
    40 μM
    Compound: 8
    Antiviral activity against influenza A virus H1N1 A/PR/8/34 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect
    Antiviral activity against influenza A virus H1N1 A/PR/8/34 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect
    [PMID: 19729316]
    MDCK EC50
    61.9 μM
    Compound: 8
    Antiviral activity against influenza A virus H9N2 A/Chicken/Korea/MS96/96 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect
    Antiviral activity against influenza A virus H9N2 A/Chicken/Korea/MS96/96 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect
    [PMID: 19729316]
    MDCK EC50
    72.8 μM
    Compound: 8
    Inhibition of Influenza A virus A/PR/8/34 (H1N1) neuraminidase activity measured in infected MDCK cells using MUNANA as substrate assessed as fluorescence intensity after 48 hrs
    Inhibition of Influenza A virus A/PR/8/34 (H1N1) neuraminidase activity measured in infected MDCK cells using MUNANA as substrate assessed as fluorescence intensity after 48 hrs
    [PMID: 25927664]
    MDCK IC50
    143.3 μg/mL
    Compound: 8
    Cytotoxicity against MDCK cells
    Cytotoxicity against MDCK cells
    [PMID: 15104496]
    MDCK IC50
    24.5 μg/mL
    Compound: 8
    Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against influenza virus type A H1N1 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 15104496]
    MDCK IC50
    26.9 μg/mL
    Compound: 8
    Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against RSV Long in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 15104496]
    MDCK IC50
    35.8 μg/mL
    Compound: 8
    Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    Antiviral activity against PIV3 in MDCK cells assessed as inhibition of virus-induced cytopathic effect
    [PMID: 15104496]
    P388 ED50
    8.2 μg/mL
    Compound: Astragalin
    Cytotoxicity against mouse P388 cells
    Cytotoxicity against mouse P388 cells
    [PMID: 3404159]
    RAW264.7 IC50
    37.1 μM
    Compound: 21
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis
    [PMID: 24679441]
    RAW264.7 IC50
    40.6 μM
    Compound: 21
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA
    [PMID: 24679441]
    RAW264.7 IC50
    64 μM
    Compound: 9
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    [PMID: 21353543]
    RAW264.7 IC50
    67.1 μM
    Compound: 9
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs
    [PMID: 21353543]
    SK-MEL-2 IC50
    > 10 μM
    Compound: 6
    Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay
    Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay
    [PMID: 28165740]
    SK-OV-3 IC50
    > 10 μM
    Compound: 6
    Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
    Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
    [PMID: 28165740]
    SW-620 IC50
    4.6 μM
    Compound: 11
    Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay
    [PMID: 25981689]
    U-251 IC50
    37.6 μM
    Compound: 11
    Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay
    [PMID: 25981689]
    U-87MG ATCC IC50
    3.12 μM
    Compound: 11
    Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay
    Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay
    [PMID: 25981689]
    Vero IC50
    174.3 μM
    Compound: 1
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis
    [PMID: 21466157]
    In Vitro

    Astragalin (0-80 μg/mL; 24 h, 48 h, 72 h) inhibits cancer cells viability and migration at 20, 40, 80 μM, and shows no cytotoxicity to normal human colon epithelial cell lines NCM460[1].
    Astragalin (80 μg/mL; 4 h, 8 h) interrupts the NF-κB signaling pathway and inhibits NF-κB P65 transcriptional activity in HCT116 cells stimulated by TNF-α[1].
    Astragalin (20, 40, 80 μg/mL; 48 h) induces HCT116 cells apoptosis and arrests cell cycle at G0/G1 phase[1].
    Astragalin (20, 40, 80 μg/mL; 48 h) increases the level of apoptin in HCT116 and decreases the level of anti-apoptotic proteins in a dose-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Human colon cancer cell lines (HCT116, LoVo, SW620, SW480, Caco2) and human colon epithelial cell lines NCM460
    Concentration: 0, 5, 10, 20, 40, 80 μg/mL
    Incubation Time: 24, 48, 72 hours
    Result: Inhibits cancer cells (LoVo, SW620, SW480, Caco2, HCT116) activity at 20, 40, 80 μM.
    Inhibited HCT116 cells with IC50s of 121.845, 87.908, 18.883 μg/mL at 24, 48, 72 hours, respectively.
    Showed no cytotoxicity with NCM460 cells.

    Cell Cycle Analysis[1]

    Cell Line: Human colon cancer cell HCT116
    Concentration: 0, 20, 40, 80 μg/mL
    Incubation Time: 48 hours
    Result: Arrested cell cycle at G0/G1.

    Western Blot Analysis[1]

    Cell Line: Human colon cancer cell HCT116
    Concentration: 80 μg/mL; pre-treated with 20 ng/mL TNF-α
    Incubation Time: 4 or 8 hours
    Result: Increased the expression of pro-apoptotic proteins (caspase 3, caspase 6, caspase 7, caspase 8, caspase 9, P53, and Bax) and decreased the expression of anti-apoptotic proteins (cleaved caspase-3 and Bcl-2) in a dose-dependent manner.

    Cell Migration Assay [1]

    Cell Line: Human colon cancer cell HCT116
    Concentration: 0, 20, 40, 80 μg/mL
    Incubation Time: 24, 48, 72 hours
    Result: Reduced release of MMP-2 and MMP-9 enzymes.
    Inhibited cell migration with mobilities of 14.38, 13.44, and 5.29% with 20, 40, and 80 μg/mL concentrations, respectively.
    In Vivo

    Astragalin (25, 50, and 75 mg/kg; p.o.; once every two days; 25 d) reduces the tumor volumes, tumor weight and tumor formation rates and decreases expression of p-NFκB and p-iκκα in nude mouse model with human colon cancer[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BALB/c nude mice (4-week-old) with HCT116 cells (s.c.)[1]
    Dosage: 0 mg/kg, 25 mg/kg, 50 mg/kg, 75 mg/kg
    Administration: Oral gavage; once every two days; for 25 days
    Result: Resulted tumor inhibition rates of 17.43%, 34.89% (p < 0.01), and 67.06% with 25 mg/kg, 50 mg/kg, 75 mg/kg dose administration.
    Showed no effect on mouse weight.
    Molecular Weight

    448.38

    Formula

    C21H20O11

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    OC1=CC2=C(C(C(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)=C(C4=CC=C(O)C=C4)O2)=O)C(O)=C1

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (223.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2303 mL 11.1513 mL 22.3025 mL
    5 mM 0.4461 mL 2.2303 mL 4.4605 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  1% CMC-Na/saline water

      Solubility: 10 mg/mL (22.30 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2303 mL 11.1513 mL 22.3025 mL 55.7563 mL
    5 mM 0.4461 mL 2.2303 mL 4.4605 mL 11.1513 mL
    10 mM 0.2230 mL 1.1151 mL 2.2303 mL 5.5756 mL
    15 mM 0.1487 mL 0.7434 mL 1.4868 mL 3.7171 mL
    20 mM 0.1115 mL 0.5576 mL 1.1151 mL 2.7878 mL
    25 mM 0.0892 mL 0.4461 mL 0.8921 mL 2.2303 mL
    30 mM 0.0743 mL 0.3717 mL 0.7434 mL 1.8585 mL
    40 mM 0.0558 mL 0.2788 mL 0.5576 mL 1.3939 mL
    50 mM 0.0446 mL 0.2230 mL 0.4461 mL 1.1151 mL
    60 mM 0.0372 mL 0.1859 mL 0.3717 mL 0.9293 mL
    80 mM 0.0279 mL 0.1394 mL 0.2788 mL 0.6970 mL
    100 mM 0.0223 mL 0.1115 mL 0.2230 mL 0.5576 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Astragalin
    Cat. No.:
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