1. GPCR/G Protein
    Immunology/Inflammation
    Apoptosis
  2. CXCR
    Apoptosis
  3. Baohuoside I

Baohuoside I (Synonyms: Icariin-II; Icariside-II)

Cat. No.: HY-N0011 Purity: 99.70%
Handling Instructions

Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

For research use only. We do not sell to patients.

Baohuoside I Chemical Structure

Baohuoside I Chemical Structure

CAS No. : 113558-15-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 158 In-stock
Estimated Time of Arrival: December 31
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
50 mg USD 576 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Baohuoside I purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366.

    Ki67 Immunofluorescence following HepG2 and Huh7 cells incubated with Baohuoside-1 or an equal volume of DMEM medium for 24 h.Baohuoside-1 inhibited proliferation in HepG2 and Huh7 cells of liver cancer, and the proliferation inhibited by Baohuoside-1 is dose dependent.

    Baohuoside I purchased from MCE. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366.

    After 24 h treatment with Baohuoside-1 (20 μM, 50 μM), the expression of metastasis-associated proteins is detected by western blotting analysis.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

    IC50 & Target

    CXCR4

     

    In Vitro

    Baohuoside I is an inhibitor of CXCR4, and downregulates CXCR4 expression at 12-25 μM. Baohuoside I (0-25 μM) suppresses NF-κB activation in a dose-dependent manner, suppresses CXCL12 induced the invasion of cervical cancer cells. Baohuoside I also inhibits invasion of breast cancer cells[1]. Baohuoside I inhibits A549 cell viability, with IC50s of 25.1 μM at 24 h, 11.5 μM and 9.6 μM at 48 h and 72 h, respectively. Baohuoside I ((25 μM) suppresses the caspase cascade in A549 cells, elevates ROS levels and activates JNK and p38MAPK signaling cascade[2]. Baohuoside I (3.125, 6.25, 12.5, 25.0 and 50.0 µg/mL) significantly and dose-dependently blocks the growth of esophageal squamous cell carcinoma Eca109 cells, with an IC50 of 4.8 µg/mL at 48 h[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Baohuoside I (25 mg/kg) decreases β-catenin protein levels, cyclin D1 and survivin expression in nude mice[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    514.52

    Formula

    C₂₇H₃₀O₁₀

    CAS No.

    113558-15-9

    SMILES

    OC1=CC(O)=C2C(OC(C3=CC=C(OC)C=C3)=C(O[[email protected]](O[[email protected]@H](C)[[email protected]](O)[[email protected]]4O)[[email protected]@H]4O)C2=O)=C1C/C=C(C)\C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 32 mg/mL (62.19 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9436 mL 9.7178 mL 19.4356 mL
    5 mM 0.3887 mL 1.9436 mL 3.8871 mL
    10 mM 0.1944 mL 0.9718 mL 1.9436 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    The cytotoxicity effect of Baohuoside I on A549 cells is determined by MTT assay. Cells (1×104 cells/well) are seeded in a 96-well plate, and treated with Baohuoside I (6.25, 12.5, and 25 μM) or 1 mM NAC for 24, 48 or 72 h. After MTT containing medium is removed, the crystals that have formed are dissolved by the addition of DMSO to each well. After mixing, the absorbance of the cells is measured at 540 nm by using Multiskan Spectrum Microplate Reader[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Mice[3]
    Female Balb/c nude mice (4- to 6-weeks-old) are used in the assay. Subconfluent Eca109-Luc cells are harvested and resuspended in PBS to a final density of 2 × 107 cells/mL. Prior to injection, cells are resuspended in PBS and analyzed by 0.4% trypan blue exclusion assay (viable cells >90%). For subcutaneous injection, 1 × 107 Eca109-Luc cells in 200 µL PBS are injected into the left flank of each mouse using 27G needles. At 1 week after tumor cell injection, Baohuoside I (25 mg/kg per mouse) is injected intralesionally once a day, whereas the 10 mice intended for vehicle treatment are administered an equal volume of PBS[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.70%

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    Keywords:

    Baohuoside IIcariin-II Icariside-IICXCRApoptosisCXC chemokine receptorsC-X-C motif chemokine receptorsInhibitorinhibitorinhibit

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