Cat. No.: HY-N0011

Baohuoside I

(Synonyms: Icariin-II; Icariside-II)

18 Cited Publications

Customer Review

Based on 18 publication(s) in Google Scholar

Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 113558-15-9
Formula: C₂₇H₃₀O₁₀
Molecular Weight:514.52

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Baohuoside I Chemical Structure

CAS No.: 113558-15-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

IC₅₀ & Target^[1]

CXCR4

All CXCR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	7.52 μM Compound: ICS II	Antitumor activity against human MCF7 cells assessed as cell viability incubated for 48 hrs by CCK8 assay Antitumor activity against human MCF7 cells assessed as cell viability incubated for 48 hrs by CCK8 assay	[PMID: 36753987]
NCI/ADR-RES	IC₅₀	>100 μM Compound: 2	Cytotoxicity against human MCF7/ADR cells after 72 hrs by MTT assay Cytotoxicity against human MCF7/ADR cells after 72 hrs by MTT assay	[PMID: 19523827]
Sf9	IC₅₀	1.7 μM Compound: 58	Inhibition of recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate incubated for 15 mins by liquid scintillation counting method Inhibition of recombinant human PDE5A1 catalytic domain (535 to 860 residues) expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate incubated for 15 mins by liquid scintillation counting method	[PMID: 26908025]
Vero	IC₅₀	9.34 μM Compound: ICS II	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 36753987]

In Vitro

Baohuoside I is an inhibitor of CXCR4, and downregulates CXCR4 expression at 12-25 μM. Baohuoside I (0-25 μM) suppresses NF-κB activation in a dose-dependent manner, suppresses CXCL12 induced the invasion of cervical cancer cells. Baohuoside I also inhibits invasion of breast cancer cells^[1]. Baohuoside I inhibits A549 cell viability, with IC₅₀s of 25.1 μM at 24 h, 11.5 μM and 9.6 μM at 48 h and 72 h, respectively. Baohuoside I ((25 μM) suppresses the caspase cascade in A549 cells, elevates ROS levels and activates JNK and p38^MAPK signaling cascade^[2]. Baohuoside I (3.125, 6.25, 12.5, 25.0 and 50.0 μg/mL) significantly and dose-dependently blocks the growth of esophageal squamous cell carcinoma Eca109 cells, with an IC₅₀ of 4.8 μg/mL at 48 h^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Baohuoside I (25 mg/kg) decreases β-catenin protein levels, cyclin D1 and survivin expression in nude mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 113558-15-9
Appearance Solid
Molecular Weight 514.52
Formula C₂₇H₃₀O₁₀
Color Light yellow to yellow
SMILES

OC1=CC(O)=C2C(OC(C3=CC=C(OC)C=C3)=C(O[C@H](O[C@@H](C)[C@H](O)[C@H]4O)[C@@H]4O)C2=O)=C1C/C=C(C)\C
Synonyms

Icariin-II; Icariside-II
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (18)

All IFWBCell Proliferation/Viability AssayCell Migration/Invasion AssayFlow CytometryHistological Imaging/StainingIn Vivo Efficacy Study

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Journal Impact Factor

Bioact Mater

A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming. [Abstract]

2025 Jun 5:52:73-91. PMID: 40530415
Adv Funct Mater

2024 Dec 23.
Small

2026 Jan 23.
Phytomedicine

Network pharmacology and experimental validation of inflammation inhibition by ChuanKeZhi Injection in treating asthma. [Abstract]

2025 May 21:143:156891. PMID: 40450983
Phytomedicine

Baohuoside I inhibits virulence of multidrug-resistant Staphylococcus aureus by targeting the transcription Staphylococcus accessory regulator factor SarZ. [Abstract]

2024 Jul 25:130:155590. PMID: 38810547
Phytomedicine

Icariside II potentiates the anti-PD-1 antitumor effect by reducing chemotactic infiltration of myeloid-derived suppressor cells into the tumor microenvironment via ROS-mediated inactivation of the SRC/ERK/STAT3 signaling pathways. [Abstract]

2023 Feb:110:154638. PMID: 36621167
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Phytother Res

Icariside II prevents kidney fibrosis development in chronic kidney disease by promoting fatty acid oxidation. [Abstract]

2024 Feb;38(2):839-855. PMID: 38081477
Cells

Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]

2026 Feb 3;15(3):287. PMID: 41677650
Front Pharmacol

Icariside Ⅱ Attenuates Palmitic Acid-Induced Endothelial Dysfunction Through SRPK1-Akt-eNOS Signaling Pathway. [Abstract]

2022 Jun 30;13:920601. PMID: 35846993
J Cell Mol Med

2020;00:1-15.
Drug Dev Res

Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR-144-3p/IGF2R axis. [Abstract]

2022 Sep;83(6):1383-1393. PMID: 35808943
J Pharm Pharmacol

Icariside I reduces breast cancer proliferation, apoptosis, invasion, and metastasis probably through inhibiting IL-6/STAT3 signaling pathway. [Abstract]

2024 May 3;76(5):499-513. PMID: 37971302
In Vitro Cell Dev Biol Anim

Icariside II inhibits ferroptosis and improves high-glucose-induced podocytes injury by downregulating DNMT1. [Abstract]

2025 Dec 22. PMID: 41428160
Rev Bras Farm

2024 Aug 30.
Biomed Pharmacother

Baohuoside-1 targeting mTOR inducing apoptsis to inhibit hepatocellular carcinoma proliferation, invasion and migration. [Abstract]

2020 Aug;128:110366. PMID: 32526459

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

Ki67 Immunofluorescence following HepG2 and Huh7 cells incubated with Baohuoside-1 or an equal volume of DMEM medium for 24 h.Baohuoside-1 inhibited proliferation in HepG2 and Huh7 cells of liver cancer, and the proliferation inhibited by Baohuoside-1 is dose dependent.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

After 24 h treatment with Baohuoside-1 (20 μM, 50 μM), the expression of metastasis-associated proteins is detected by western blotting analysis.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

CCK8 assay following HepG2 and Huh7 incubated with 5 μM,10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 90 μM Baohuoside-1 or an equal volume of DMEM medium for 24 h.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

Transwell assay following HepG2 and Huh7 cells incubated with Baohuoside-1 (20 μM, 50 μM) or an equal volume of DMEM medium for 24 h.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

Flow cytometry for apoptosis [apoptosis ratio was calculated as (Q2 + Q3)/(Q1+Q2 + Q3+Q4)] of HepG2 and Huh7 cells incubated with Baohuoside-1 (20 μM, 50 μM) or an equal volume of DMEM medium for 24 h.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

HE showed that Baohuoside-1 (25 mg/kg, i.g.) significantly inhibits tumor growth of cell xenografts in nude mice.

Baohuoside I purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Aug;128:110366. [Abstract]

The tumor volumes were measured every three day for 30 days treated with Baohuoside I (25 mg/kg, i.g.).
Biomed Pharmacother

Developing neobavaisoflavone nanoemulsion suppresses lung cancer progression by regulating tumor microenvironment. [Abstract]

2020 Sep;129:110369. PMID: 32563983

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (62.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9436 mL

9.7178 mL

19.4356 mL

5 mM

0.3887 mL

1.9436 mL

3.8871 mL

10 mM

0.1944 mL

0.9718 mL

1.9436 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC/saline water

Solubility: 20 mg/mL (38.87 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

The cytotoxicity effect of Baohuoside I on A549 cells is determined by MTT assay. Cells (1×10⁴ cells/well) are seeded in a 96-well plate, and treated with Baohuoside I (6.25, 12.5, and 25 μM) or 1 mM NAC for 24, 48 or 72 h. After MTT containing medium is removed, the crystals that have formed are dissolved by the addition of DMSO to each well. After mixing, the absorbance of the cells is measured at 540 nm by using Multiskan Spectrum Microplate Reader^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Mice^[3]
Female Balb/c nude mice (4- to 6-weeks-old) are used in the assay. Subconfluent Eca109-Luc cells are harvested and resuspended in PBS to a final density of 2 × 10⁷ cells/mL. Prior to injection, cells are resuspended in PBS and analyzed by 0.4% trypan blue exclusion assay (viable cells >90%). For subcutaneous injection, 1 × 10⁷ Eca109-Luc cells in 200 µL PBS are injected into the left flank of each mouse using 27G needles. At 1 week after tumor cell injection, Baohuoside I (25 mg/kg per mouse) is injected intralesionally once a day, whereas the 10 mice intended for vehicle treatment are administered an equal volume of PBS^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim B, et al. Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR4 chemokine receptor expression. Biochemistry. 2014 Dec 9;53(48):7562-9. [Content Brief]

[2]. Song J, et al. Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer. Chem Biol Interact. 2012 Jul 30;199(1):9-17. [Content Brief]

[3]. Wang L, et al. The flavonoid Baohuoside-I inhibits cell growth and downregulates survivin and cyclin D1 expression in esophageal carcinoma via β-catenin-dependent signaling. Oncol Rep. 2011 Nov;26(5):1149-56. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9436 mL	9.7178 mL	19.4356 mL	48.5890 mL
	5 mM	0.3887 mL	1.9436 mL	3.8871 mL	9.7178 mL
	10 mM	0.1944 mL	0.9718 mL	1.9436 mL	4.8589 mL
	15 mM	0.1296 mL	0.6479 mL	1.2957 mL	3.2393 mL
	20 mM	0.0972 mL	0.4859 mL	0.9718 mL	2.4294 mL
	25 mM	0.0777 mL	0.3887 mL	0.7774 mL	1.9436 mL
	30 mM	0.0648 mL	0.3239 mL	0.6479 mL	1.6196 mL
	40 mM	0.0486 mL	0.2429 mL	0.4859 mL	1.2147 mL
	50 mM	0.0389 mL	0.1944 mL	0.3887 mL	0.9718 mL
	60 mM	0.0324 mL	0.1620 mL	0.3239 mL	0.8098 mL

Keywords

Baohuoside I

18 Cited Publications

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Baohuoside I

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Publications Citing Use of MedChemExpress (MCE) Baohuoside I

All CXCR Isoforms

Biological Activity

Chemical Information

Publications (18)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Baohuoside I Related Classifications

HY-N0011 Related Classifications