1. GPCR/G Protein
    Immunology/Inflammation
    Apoptosis
  2. CXCR
    Apoptosis

Baohuoside I (Synonyms: Icariin-II; Icariside-II)

Cat. No.: HY-N0011 Purity: 98.96%
Handling Instructions

Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

For research use only. We do not sell to patients.

Baohuoside I Chemical Structure

Baohuoside I Chemical Structure

CAS No. : 113558-15-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 158 In-stock
Estimated Time of Arrival: December 31
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
50 mg USD 576 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.

IC50 & Target[1]

CXCR4

 

Apoptosis

 

In Vitro

Baohuoside I is an inhibitor of CXCR4, and downregulates CXCR4 expression at 12-25 μM. Baohuoside I (0-25 μM) suppresses NF-κB activation in a dose-dependent manner, suppresses CXCL12 induced the invasion of cervical cancer cells. Baohuoside I also inhibits invasion of breast cancer cells[1]. Baohuoside I inhibits A549 cell viability, with IC50s of 25.1 μM at 24 h, 11.5 μM and 9.6 μM at 48 h and 72 h, respectively. Baohuoside I ((25 μM) suppresses the caspase cascade in A549 cells, elevates ROS levels and activates JNK and p38MAPK signaling cascade[2]. Baohuoside I (3.125, 6.25, 12.5, 25.0 and 50.0 µg/mL) significantly and dose-dependently blocks the growth of esophageal squamous cell carcinoma Eca109 cells, with an IC50 of 4.8 µg/mL at 48 h[3].

In Vivo

Baohuoside I (25 mg/kg) decreases β-catenin protein levels, cyclin D1 and survivin expression in nude mice[3].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 32 mg/mL (62.19 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9436 mL 9.7178 mL 19.4356 mL
5 mM 0.3887 mL 1.9436 mL 3.8871 mL
10 mM 0.1944 mL 0.9718 mL 1.9436 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

The cytotoxicity effect of Baohuoside I on A549 cells is determined by MTT assay. Cells (1×104 cells/well) are seeded in a 96-well plate, and treated with Baohuoside I (6.25, 12.5, and 25 μM) or 1 mM NAC for 24, 48 or 72 h. After MTT containing medium is removed, the crystals that have formed are dissolved by the addition of DMSO to each well. After mixing, the absorbance of the cells is measured at 540 nm by using Multiskan Spectrum Microplate Reader[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
Female Balb/c nude mice (4- to 6-weeks-old) are used in the assay. Subconfluent Eca109-Luc cells are harvested and resuspended in PBS to a final density of 2 × 107 cells/mL. Prior to injection, cells are resuspended in PBS and analyzed by 0.4% trypan blue exclusion assay (viable cells >90%). For subcutaneous injection, 1 × 107 Eca109-Luc cells in 200 µL PBS are injected into the left flank of each mouse using 27G needles. At 1 week after tumor cell injection, Baohuoside I (25 mg/kg per mouse) is injected intralesionally once a day, whereas the 10 mice intended for vehicle treatment are administered an equal volume of PBS[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

514.52

Formula

C₂₇H₃₀O₁₀

CAS No.

113558-15-9

SMILES

OC1=CC(O)=C2C(OC(C3=CC=C(OC)C=C3)=C(O[[email protected]](O[[email protected]@H](C)[[email protected]](O)[[email protected]]4O)[[email protected]@H]4O)C2=O)=C1C/C=C(C)\C

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Baohuoside I
Cat. No.:
HY-N0011
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Baohuoside I

Cat. No.: HY-N0011