2. Platycodin D

Platycodin D is a saponin isolated from Platycodon grandiflorus, acts as an activator of AMPKα, with anti-obesity property. WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D.

For research use only. We do not sell to patients.

Platycodin D Chemical Structure

Platycodin D Chemical Structure

CAS No. : 58479-68-8

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 110 In-stock
20 mg USD 198 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 8 publication(s) in Google Scholar

Platycodin D Related Antibodies

Top Publications Citing Use of Products

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb.

    Platycodin D (PlaD; 3.75, 5, 7.5, 10, 15, 20 μM; 24 h) dose-dependently inhibits the viability of dHL-60 cells.

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb.

    Platycodin D (PlaD; 4, 8, 12 μM) inhibits the high expression of PD-L1 in dHL-60 cells induced by IFN-γ (Fig. F and G).

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb.

    When the dHL-60 cells are treated with Platycodin D (PlaD; 0, 4, 8, 12 μM; 24 h), the numbers of migrated cells decreases significantly in a dose-dependent manner with or without IFN-γ induction.

    Platycodin D purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2023 Feb.

    Platycodin D (PlaD; 10, 20 mg/kg; i.g; once daily for 23 days) significantly inhibited tumor growth (fig B).The tumor inhibition rate is approximately 39 % in response to 10 mg/kg and 57 % in response to 20 mg/kg (fig C).

    • Biological Activity

    • Purity & Documentation

    • Customer Review

    Description

    Platycodin D is a saponin isolated from Platycodon grandiflorus, acts as an activator of AMPKα, with anti-obesity property. WNT/β-catenin pathway mediates the anti-adipogenic effect of platycodin D[1][2].

    IC50 & Target

    AMPKα[1]
    In Vitro

    Platycodin D (4 μM; 24 h) increases gene expressions of Ucp1, Pgc1a, Sirt3, Cytc, Nrf1 and Prdm16, and suppresses expressions of adipokine genes Fabp4, Adipoq, Lipin1 in 3T3-L1 adipocytes and FABP4, ADIPOQ and RESISTIN in hAMSCs[1].
    Platycodin D (2 and 10 μM) suppresses lipid accumulation in murine and human adipocytes respectively[1].
    Platycodin D suppresses the protein expressions of PPARγ and C/EBPα, and increases protein expressions of UCP1, PGC1α, SIRT3, CIDEA and LIPIN1[1].
    Platycodin D (10 μM) inhibits lipid droplet formation, and reduces the expression of the FABP4 protein involved in lipid metabolism and the expressions of PPARγ and C/EBPα[2].
    Platycodin D (10 μM; 0-7 d) recoveres CCND1 mRNA level and increases PPARδ level[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Platycodin D Related Antibodies
    In Vivo

    Platycodin D (5 mg/kg; oral administration, 5 times per week for 5 weeks) decreases the body weight of obese mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Genetically obese db/db mice[1]
    Dosage: 5 mg/kg
    Administration: Oral administration; 5 mg/kg, 5 times per week for 5 weeks
    Result: Significantly decreased body weight gain without affecting food intake of mice. Decreased the liver tissue weight and increased the weight of brown adipose tissue (BAT).
    Molecular Weight

    1225.32

    Formula

    C57H92O28
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C([C@]1(CCC(C)(C)C2)[C@]2([H])C3=CC[C@@]4([H])[C@@](C)(CC[C@]5([H])[C@@]4(C[C@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]6O)O)([H])O[C@@H]6CO)C5(CO)CO)C)[C@]3(C)C[C@H]1O)O[C@H](OC[C@H](O)[C@@H]7O)[C@@H]7O[C@@](O[C@@H](C)[C@H](O[C@@](OC[C@@H](O)[C@@H]8O[C@@](OC[C@]9(O)CO)([H])[C@@H]9O)([H])[C@@H]8O)[C@H]%10O)([H])[C@@H]%10O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (40.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8161 mL 4.0806 mL 8.1611 mL
    5 mM 0.1632 mL 0.8161 mL 1.6322 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (2.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (2.04 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 20 mg/mL (16.32 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 20 mg/mL (16.32 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.80%

    COA (254 KB) HNMR (366 KB) LCMS (264 KB)

    Handling Instructions (2659 KB)

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.8161 mL 4.0806 mL 8.1611 mL 20.4028 mL
    5 mM 0.1632 mL 0.8161 mL 1.6322 mL 4.0806 mL
    10 mM 0.0816 mL 0.4081 mL 0.8161 mL 2.0403 mL
    15 mM 0.0544 mL 0.2720 mL 0.5441 mL 1.3602 mL
    20 mM 0.0408 mL 0.2040 mL 0.4081 mL 1.0201 mL
    25 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8161 mL
    30 mM 0.0272 mL 0.1360 mL 0.2720 mL 0.6801 mL
    40 mM 0.0204 mL 0.1020 mL 0.2040 mL 0.5101 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Platycodin D58479-68-8Inhibitorinhibitorinhibit

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