2. Others
  3. Fluorescent Dye
  4. Biotin NHS

Biotin NHS  (Synonyms: Biotin N-hydroxysuccinimide ester; NHS-Biotin)

Cat. No.: HY-D0802 Purity: 99.85%
COA
SDS
Handling Instructions Technical Support

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

For research use only. We do not sell to patients.

CAS No. : 35013-72-0

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
100 mg In-stock
200 mg In-stock
500 mg   Get quote  
1 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 19 publication(s) in Google Scholar

Biotin NHS Related Antibodies

Top Publications Citing Use of Products

    Biotin NHS purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Sep 29:35:102365.  [Abstract]

    Schematic for fabrication of Protein-Biotin and Protein-Biotin HAG using Biotin-NHS (The protein and Biotin-NHS solutions were mixed at a molar ratio of 1:5 and allowed to react for 0.5 h at 4 °C to form Protein-Biotin conjugates.).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Buffer Exchange: Ensure the protein is in an amine-free buffer (e.g., PBS, 100 mM sodium phosphate, 150 mM NaCl) at pH 7.2-7.5. Avoid Tris or glycine.
    2. Reagent Prep: Equilibrate the NHS-Biotin vial to room temperature before opening to avoid condensation. Dissolve the reagent in high-quality anhydrous DMSO or DMF immediately before use.
    3. Reaction Ratio: Use a 12-20 fold molar excess of biotin reagent for protein concentrations of 2-10 mg/mL.
    4. Incubation: Incubate for 30-60 minutes at room temperature, or 2 hours at 4°C.
    5. Purification: Remove unreacted biotin using desalting columns (e.g., Sephadex G-25) or dialysis.
    6. Verification: Estimate incorporation using the HABA assay, where biotin displaces HABA from avidin, decreasing absorbance at 500nm.
    Note:
    1. Sulfo-NHS vs. NHS: Sulfo-NHS-Biotin is water-soluble and ideal for aqueous reactions, while NHS-Biotin requires organic solvent dissolution first.
    2. Moisture Sensitivity: NHS reagents are highly moisture-sensitive; store desiccated at ?20°C (or 4°C for some LC-NHS) and protect from humidity.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Biotin NHS Related Antibodies
    Molecular Weight

    341.38

    Formula

    C14H19N3O5S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(ON1C(CCC1=O)=O)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 62.5 mg/mL (183.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9293 mL 14.6464 mL 29.2929 mL
    5 mM 0.5859 mL 2.9293 mL 5.8586 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.85%

    SDS (393 KB)

    COA (249 KB) HNMR (253 KB) RP-HPLC (238 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9293 mL 14.6464 mL 29.2929 mL 73.2322 mL
    5 mM 0.5859 mL 2.9293 mL 5.8586 mL 14.6464 mL
    10 mM 0.2929 mL 1.4646 mL 2.9293 mL 7.3232 mL
    15 mM 0.1953 mL 0.9764 mL 1.9529 mL 4.8821 mL
    20 mM 0.1465 mL 0.7323 mL 1.4646 mL 3.6616 mL
    25 mM 0.1172 mL 0.5859 mL 1.1717 mL 2.9293 mL
    30 mM 0.0976 mL 0.4882 mL 0.9764 mL 2.4411 mL
    40 mM 0.0732 mL 0.3662 mL 0.7323 mL 1.8308 mL
    50 mM 0.0586 mL 0.2929 mL 0.5859 mL 1.4646 mL
    60 mM 0.0488 mL 0.2441 mL 0.4882 mL 1.2205 mL
    80 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9154 mL
    100 mM 0.0293 mL 0.1465 mL 0.2929 mL 0.7323 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Biotin NHS Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Biotin NHS35013-72-0Biotin N-hydroxysuccinimide ester NHS-BiotinFluorescent DyeInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Biotin NHS
    Cat. No.:
    HY-D0802
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.