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  2. Biotin NHS

Biotin NHS (Synonyms: Biotin-NHS; Biotin N-hydroxysuccinimide ester; NHS-Biotin)

Cat. No.: HY-D0802 Purity: ≥98.0%
Handling Instructions

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

For research use only. We do not sell to patients.

Biotin NHS Chemical Structure

Biotin NHS Chemical Structure

CAS No. : 35013-72-0

Size Price Stock Quantity
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
200 mg USD 96 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
1 g   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

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Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
Biotin Labeling Reaction:
1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
5. To tests the labeled protein by ELISA or Western blot.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight





O=C(ON1C(CCC1=O)=O)CCCC[[email protected]@H]2SC[[email protected]]([[email protected]]2([H])N3)([H])NC3=O


Room temperature in continental US; may vary elsewhere.


-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : ≥ 3.4 mg/mL (9.96 mM)

*"≥" means soluble, but saturation unknown.

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9293 mL 14.6464 mL 29.2929 mL
5 mM 0.5859 mL 2.9293 mL 5.8586 mL
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.

Purity: ≥98.0%

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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2


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