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Biotin NHS  (Synonyms: Biotin-NHS; Biotin N-hydroxysuccinimide ester; NHS-Biotin)

Cat. No.: HY-D0802 Purity: ≥98.0%
COA Handling Instructions

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

For research use only. We do not sell to patients.

Biotin NHS Chemical Structure

Biotin NHS Chemical Structure

CAS No. : 35013-72-0

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200 mg USD 95 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

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Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
? Calculations:
? For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
? 1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
? mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
? * 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
? 2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
? mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
? Biotin Labeling Reaction:
? 1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
? 2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
? 3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
? 4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
? 5. To tests the labeled protein by ELISA or Western blot.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







White to off-white




Room temperature in continental US; may vary elsewhere.


-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (183.08 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9293 mL 14.6464 mL 29.2929 mL
5 mM 0.5859 mL 2.9293 mL 5.8586 mL
10 mM 0.2929 mL 1.4646 mL 2.9293 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

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Biotin NHS Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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