DBCO-NHS ester
Based on 6 publication(s) in Google Scholar
DBCO-NHS ester is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). DBCO-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 99.47%
- CAS No.: 1353016-71-3
- Formula: C23H18N2O5
- Molecular Weight:402.40
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DBCO-NHS ester
More
Customer Validation & Images
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Gel Electrophoresis
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Gel Electrophoresis
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Gel Electrophoresis
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
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Non-cleavable Linker |
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1353016-71-3
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Appearance Solid
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Molecular Weight 402.40
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Formula C23H18N2O5
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Color White to yellow
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SMILES
O=C(CCC(ON(C(CC1)=O)C1=O)=O)N(C(C=CC=C2)=C2C#C3)CC4=C3C=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Angew Chem Int Ed Engl
Adapting and Remolding: Orchestrating Tumor Microenvironment Normalization with Photodynamic Therapy by Size Transformable Nanoframeworks. [Abstract]2021 May 10;60(20):11464-11473. PMID: 33751758
DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Angew Chem Int Ed Engl. 2021 May 10;60(20):11464-11473. [Abstract]
Mass spectra of ApoFRT, ApoFRT-DBCO, and ApoFRT-pep-biotin.The small ferritin nanocage (ApoFRT) was conjugated with azide/biotin dual-modified peptide (N3-pep-biotin) via DBCO-NHS (dibenzocyclooctyne-N-hydroxysuccinimidylester) activation and click chemistry, which was verified by mass spectrometry analysis.
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Nat Commun
An in situ dual-anchoring strategy for enhanced immobilization of PD-L1 to treat autoimmune diseases. [Abstract]2023 Oct 31;14(1):6953. PMID: 37907476
DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Oct 31;14(1):6953. [Abstract]
UV-visible absorption spectra of PD-L1 (0.5 mg/mL) and DBCO-PD-L1. DBCO-NHS (150 nM; 3 μL; 20mg/mL in DMSO) ligands could be conjugated to PD-L1.
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Int J Biol Macromol
Antibody-DNA nanostructure conjugates exhibit inhibited effects on CT26 cells and prevent upregulation of PD-1 in the tumor microenvironment. [Abstract]2025 Oct 3;330(Pt 3):148101. PMID: 41046886 -
Eur J Pharm Biopharm
Multivalent mannose-conjugated siRNA causes robust gene silencing in pancreatic macrophages in vivo. [Abstract]2023 Feb:183:61-73. PMID: 36603692 -
DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Gelred staining result of BSA-coupled DNA PAGE gel under freezing.
DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Coomassie blue staining result of BSA-coupled S3 PAGE gel under freezing.
DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). The result of Gelred staining PAGE gel of BSA coupled to S3/S4 and DNA.S3: BSA coupled with 13-fold molar excess of S3. S4: BSA coupled with 13-fold molar excess of S4.
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Solvent & Solubility
DMSO : 50 mg/mL (124.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | 62.1272 mL |
| 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | 12.4254 mL | |
| 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL | 6.2127 mL | |
| 15 mM | 0.1657 mL | 0.8284 mL | 1.6567 mL | 4.1418 mL | |
| 20 mM | 0.1243 mL | 0.6213 mL | 1.2425 mL | 3.1064 mL | |
| 25 mM | 0.0994 mL | 0.4970 mL | 0.9940 mL | 2.4851 mL | |
| 30 mM | 0.0828 mL | 0.4142 mL | 0.8284 mL | 2.0709 mL | |
| 40 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5532 mL | |
| 50 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2425 mL | |
| 60 mM | 0.0414 mL | 0.2071 mL | 0.4142 mL | 1.0355 mL | |
| 80 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7766 mL | |
| 100 mM | 0.0249 mL | 0.1243 mL | 0.2485 mL | 0.6213 mL |
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