2. Antibody-drug Conjugate/ADC Related
  3. ADC Linker
  4. DBCO-NHS ester

DBCO-NHS ester is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). DBCO-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

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CAS No. : 1353016-71-3

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Based on 6 publication(s) in Google Scholar

DBCO-NHS ester Related Antibodies

Top Publications Citing Use of Products

    DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

    DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Gelred staining result of BSA-coupled DNA PAGE gel under freezing.

    DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

    DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Coomassie blue staining result of BSA-coupled S3 PAGE gel under freezing.

    DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

    DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). The result of Gelred staining PAGE gel of BSA coupled to S3/S4 and DNA.S3: BSA coupled with 13-fold molar excess of S3. S4: BSA coupled with 13-fold molar excess of S4.

    DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Oct 31;14(1):6953.  [Abstract]

    UV-visible absorption spectra of PD-L1 (0.5 mg/mL) and DBCO-PD-L1. DBCO-NHS (150 nM; 3 μL; 20mg/mL in DMSO) ligands could be conjugated to PD-L1.

    DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Angew Chem Int Ed Engl. 2021 May 10;60(20):11464-11473.  [Abstract]

    Mass spectra of ApoFRT, ApoFRT-DBCO, and ApoFRT-pep-biotin.
    The small ferritin nanocage (ApoFRT) was conjugated with azide/biotin dual-modified peptide (N3-pep-biotin) via DBCO-NHS (dibenzocyclooctyne-N-hydroxysuccinimidylester) activation and click chemistry, which was verified by mass spectrometry analysis.

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    Description

    DBCO-NHS ester is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)[1]. DBCO-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

    IC50 & Target[1]

    Non-cleavable Linker

     

    In Vitro

    ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DBCO-NHS ester Related Antibodies
    Molecular Weight

    402.40

    Formula

    C23H18N2O5
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(CCC(ON(C(CC1)=O)C1=O)=O)N(C(C=CC=C2)=C2C#C3)CC4=C3C=CC=C4
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (124.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4851 mL 12.4254 mL 24.8509 mL
    5 mM 0.4970 mL 2.4851 mL 4.9702 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    C2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

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    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.53%

    SDS (394 KB)

    COA (252 KB) HNMR (265 KB) LCMS (268 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4851 mL 12.4254 mL 24.8509 mL 62.1272 mL
    5 mM 0.4970 mL 2.4851 mL 4.9702 mL 12.4254 mL
    10 mM 0.2485 mL 1.2425 mL 2.4851 mL 6.2127 mL
    15 mM 0.1657 mL 0.8284 mL 1.6567 mL 4.1418 mL
    20 mM 0.1243 mL 0.6213 mL 1.2425 mL 3.1064 mL
    25 mM 0.0994 mL 0.4970 mL 0.9940 mL 2.4851 mL
    30 mM 0.0828 mL 0.4142 mL 0.8284 mL 2.0709 mL
    40 mM 0.0621 mL 0.3106 mL 0.6213 mL 1.5532 mL
    50 mM 0.0497 mL 0.2485 mL 0.4970 mL 1.2425 mL
    60 mM 0.0414 mL 0.2071 mL 0.4142 mL 1.0355 mL
    80 mM 0.0311 mL 0.1553 mL 0.3106 mL 0.7766 mL
    100 mM 0.0249 mL 0.1243 mL 0.2485 mL 0.6213 mL
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    DBCO-NHS ester Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DBCO-NHS ester1353016-71-3ADC LinkerAntibody-drug conjugates linkercleavableADCantibody-drugconjugatesInhibitorinhibitorinhibit

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