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Cat. No.: HY-42973

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DBCO-NHS ester

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

DBCO-NHS ester is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). DBCO-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

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Pureté: 99.47%
CAS No.: 1353016-71-3
Formule: C₂₃H₁₈N₂O₅
Masse moléculaire:402.40

Stockage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

DBCO-NHS ester Chemical Structure

CAS No.: 1353016-71-3

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

DBCO-NHS ester is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. DBCO-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC₅₀ & Target^{[1]

Non-cleavable Linker

Voir tous les produits spécifiques à Isoform ADC Linkers :

Cleavable Linker

Non-cleavable Linker

Acid Cleavable Linker

Disulfide Cleavable Linker

Protease Cleavable Linker

Glycosidase Cleavable Linker

Phosphatase Cleavable Linker}

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1353016-71-3
Appearance Solid
Masse moléculaire 402.40
Formule C₂₃H₁₈N₂O₅
Color White to yellow
SMILES

O=C(CCC(ON(C(CC1)=O)C1=O)=O)N(C(C=CC=C2)=C2C#C3)CC4=C3C=CC=C4
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

All Bio/Physico-chemical AssayGel Electrophoresis

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Journal Impact Factor

Nat Commun

An in situ dual-anchoring strategy for enhanced immobilization of PD-L1 to treat autoimmune diseases. [Abstract]

2023 Oct 31;14(1):6953. PMID: 37907476

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Oct 31;14(1):6953. [Abstract]

UV-visible absorption spectra of PD-L1 (0.5 mg/mL) and DBCO-PD-L1. DBCO-NHS (150 nM; 3 μL; 20mg/mL in DMSO) ligands could be conjugated to PD-L1.
Angew Chem Int Ed Engl

Adapting and Remolding: Orchestrating Tumor Microenvironment Normalization with Photodynamic Therapy by Size Transformable Nanoframeworks. [Abstract]

2021 May 10;60(20):11464-11473. PMID: 33751758

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Angew Chem Int Ed Engl. 2021 May 10;60(20):11464-11473. [Abstract]

Mass spectra of ApoFRT, ApoFRT-DBCO, and ApoFRT-pep-biotin.
The small ferritin nanocage (ApoFRT) was conjugated with azide/biotin dual-modified peptide (N3-pep-biotin) via DBCO-NHS (dibenzocyclooctyne-N-hydroxysuccinimidylester) activation and click chemistry, which was verified by mass spectrometry analysis.
Adv Sci (Weinh)

Light-Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction. [Abstract]

2026 May 6:e75519. PMID: 42089450
J Control Release

Biomimetic trident lipid anchoring enables stable neutrophil-based combination therapy. [Abstract]

2026 Jun 10:394:114903. PMID: 41942056
Int J Biol Macromol

Antibody-DNA nanostructure conjugates exhibit inhibited effects on CT26 cells and prevent upregulation of PD-1 in the tumor microenvironment. [Abstract]

2025 Oct 3;330(Pt 3):148101. PMID: 41046886
Eur J Pharm Biopharm

Multivalent mannose-conjugated siRNA causes robust gene silencing in pancreatic macrophages in vivo. [Abstract]

2023 Feb:183:61-73. PMID: 36603692
Patent

US20250152698A1.

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Gelred staining result of BSA-coupled DNA PAGE gel under freezing.

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Coomassie blue staining result of BSA-coupled S3 PAGE gel under freezing.

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). The result of Gelred staining PAGE gel of BSA coupled to S3/S4 and DNA.S3: BSA coupled with 13-fold molar excess of S3. S4: BSA coupled with 13-fold molar excess of S4.
Brno University of Technology

2024.

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (124.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4851 mL

12.4254 mL

24.8509 mL

5 mM

0.4970 mL

2.4851 mL

4.9702 mL

10 mM

0.2485 mL

1.2425 mL

2.4851 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (274 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Method for identifying potential drug target protein useful for development of antibody drug, and method for producing antibody directed against target protein. WO2018151301A1.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4851 mL	12.4254 mL	24.8509 mL	62.1272 mL
	5 mM	0.4970 mL	2.4851 mL	4.9702 mL	12.4254 mL
	10 mM	0.2485 mL	1.2425 mL	2.4851 mL	6.2127 mL
	15 mM	0.1657 mL	0.8284 mL	1.6567 mL	4.1418 mL
	20 mM	0.1243 mL	0.6213 mL	1.2425 mL	3.1064 mL
	25 mM	0.0994 mL	0.4970 mL	0.9940 mL	2.4851 mL
	30 mM	0.0828 mL	0.4142 mL	0.8284 mL	2.0709 mL
	40 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5532 mL
	50 mM	0.0497 mL	0.2485 mL	0.4970 mL	1.2425 mL
	60 mM	0.0414 mL	0.2071 mL	0.4142 mL	1.0355 mL
	80 mM	0.0311 mL	0.1553 mL	0.3106 mL	0.7766 mL
	100 mM	0.0249 mL	0.1243 mL	0.2485 mL	0.6213 mL

Keywords

DBCO-NHS ester

8 Cited Publications

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références

1/16
1/16

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

Customer Validation
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Gelred staining result of BSA-coupled DNA PAGE gel under freezing.
1/16

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

Customer Validation
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). Coomassie blue staining result of BSA-coupled S3 PAGE gel under freezing.
1/16

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Patent. US20250152698A1.

Customer Validation
DBCO-NHS (Dibenzocyclooctyne-N-hydroxyuccinimide) (2.8-3.3 mM; room temperature for 1 h under shaking; freezing at -20 ℃ overnight). The result of Gelred staining PAGE gel of BSA coupled to S3/S4 and DNA.S3: BSA coupled with 13-fold molar excess of S3. S4: BSA coupled with 13-fold molar excess of S4.
1/16

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Oct 31;14(1):6953. [Abstract]

Customer Validation
UV-visible absorption spectra of PD-L1 (0.5 mg/mL) and DBCO-PD-L1. DBCO-NHS (150 nM; 3 μL; 20mg/mL in DMSO) ligands could be conjugated to PD-L1.
1/16

DBCO-NHS ester purchased from MedChemExpress. Usage Cited in: Angew Chem Int Ed Engl. 2021 May 10;60(20):11464-11473. [Abstract]

Customer Validation
Mass spectra of ApoFRT, ApoFRT-DBCO, and ApoFRT-pep-biotin.
The small ferritin nanocage (ApoFRT) was conjugated with azide/biotin dual-modified peptide (N3-pep-biotin) via DBCO-NHS (dibenzocyclooctyne-N-hydroxysuccinimidylester) activation and click chemistry, which was verified by mass spectrometry analysis.

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DBCO-NHS ester

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) DBCO-NHS ester

Voir tous les produits spécifiques à Isoform ADC Linkers

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

DBCO-NHS ester

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) DBCO-NHS ester

Voir tous les produits spécifiques à Isoform ADC Linkers

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

DBCO-NHS ester Related Classifications

HY-42973 Related Classifications