DBCO-PEG1-NHS ester
Based on 1 Customer Validation
DBCO-PEG1-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-PEG1-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 2228857-34-7
- Formula: C28H27N3O7
- Molecular Weight:517.53
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-42973
DBCO-NHS esterADC Linker -
HY-140272
DBCO-PEG4-NHS esterPROTAC Linker -
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DBCO-PEG24-NHS esterClick Chemical Agent -
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DBCO-PEG5-NHS esterADC/PROTAC Linker -
HY-170528
DBCO-PEG3-NHSPROTAC Linker -
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DBCO-PEG2-NHS esterAzide Compound -
HY-42972
DBCO-acidClick Chemistry Reagent -
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DBCO-NHCO-PEG5-NHS esterPROTAC Linker -
HY-115524
DBCO-NHS ester 2ADC linker -
HY-123687
DBCO-Sulfo-NHS ester sodiumADC Linker -
HY-133413
DBCO-CONH-S-S-NHS esterADC Linker -
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DBCO-C6-acidADC Linker
All PROTAC Linkers Isoforms
More
Biological Activity
Description
IC50 & Target
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PEGs |
In Vitro
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2228857-34-7
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Appearance Solid
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Molecular Weight 517.53
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Formula C28H27N3O7
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Color Off-white to yellow
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SMILES
O=C(N1C2=CC=CC=C2C#CC3=CC=CC=C3C1)CCC(NCCOCCC(ON(C4=O)C(CC4)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DBCO-PEG1-NHS ester Related Classifications
HY-140271 Related Classifications
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