Ac4ManNAz (80% α isomer)
Based on 7 publication(s) in Google Scholar
Ac4ManNAz is an azido-containing metabolic glycoprotein labeling reagent. Ac4ManNAz can be used to selectively modify proteins. Ac4ManNAz can be used in cell labeling, tracking and proteomic analysis. Ac4ManNAz is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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- Pureté : 99.74%
- CAS No.: 361154-30-5
- Formule: C16H22N4O10
- Masse moléculaire:430.37
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Ac4ManNAz (80% α isomer)
More- Cell Host Microbe. 2026 Feb 11;34(2):212-229.e7. [Abstract]
- Adv Funct Mater. 2023 Jan 27.
- Nat Commun. 2023 Oct 31;14(1):6953. [Abstract]
- Adv Sci (Weinh). 2026 May 20:e75770. [Abstract]
- J Control Release. 2025 Oct 14;388(Pt 1):114321. [Abstract]
- ACS Appl Mater Interfaces. 2024 Jul 17;16(28):35874-35886. [Abstract]
- SSRN. 2026 May 16.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
Activité biologique
Description
In Vitro
Ac4ManNAz (10 μM) shows the least effect on cellular systems and has a sufficient labeling efficiency for cell labeling, tracking and proteomic analysis[1].
Ac4ManNAz (50 μM) leads to the reduction of major cellular functions, including energy generation capacity, cellular infiltration ability and channel activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 361154-30-5
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Appearance Solid
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Masse moléculaire 430.37
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Formule C16H22N4O10
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Color White to light yellow
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SMILES
O=C(N[C@@H]1C(OC(C)=O)O[C@H](COC(C)=O)[C@@H](OC(C)=O)[C@@H]1OC(C)=O)CN=[N+]=[N-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (7)
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Journal Impact Factor
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Most Recent
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Cell Host Microbe
Engineered probiotics recruit CAR macrophages and establish immune memory to eradicate heterogeneous glioblastoma in mice. [Abstract]2026 Feb 11;34(2):212-229.e7. PMID: 41605215 -
Ac4ManNAz (80% α isomer) purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2023 Jan 27.
Relative cell viability of NIH3T3 cells after the Ac4ManNAz (5-50 μM; 2 days) treatment as evaluated by CCK assay. n = 5 per group.
Ac4ManNAz (80% α isomer) purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2023 Jan 27.
Bright field images of NIH3T3 cells after the Ac4ManNAz (5-50 μM; 2 days) treatment at various concentration. Scale bars = 100 µm. n = 6 per group.
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Nat Commun
An in situ dual-anchoring strategy for enhanced immobilization of PD-L1 to treat autoimmune diseases. [Abstract]2023 Oct 31;14(1):6953. PMID: 37907476 -
Adv Sci (Weinh)
Senescent Cell Derived Artificial Vesicle-Based Senolytic Sonovaccine Platform with Augmented Lymph Node Delivery and Antigen Cross-Presentation Efficacy. [Abstract]2026 May 20:e75770. PMID: 42159453 -
J Control Release
Precisely targeting of engineered nanovesicles to implanted scaffolds via click chemistry for microenvironment regulation in acute spinal cord injury. [Abstract]2025 Oct 14;388(Pt 1):114321. PMID: 41101695
Ac4ManNAz (80% α isomer) purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Oct 14;388(Pt 1):114321. [Abstract]
Viability of MSCs treated with different concentrations of Ac4ManNAz (15-60 μM; 24 h) (n = 3).
Ac4ManNAz (80% α isomer) purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Oct 14;388(Pt 1):114321. [Abstract]
Viability of MSCs treated with different concentrations of Ac4ManNAz (15-60 μM; 24 h) (n = 3).
Ac4ManNAz (80% α isomer) purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Oct 14;388(Pt 1):114321. [Abstract]
Ac4ManNAz (30 μM; 12 h) maximized the modification efficiency of azide groups on the membranes of MSCs (aMSCs), thereby enabling the efficient production of azide-functionalized aNVs.
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ACS Appl Mater Interfaces
Integrating Metabolic Oligosaccharide Engineering and SPAAC Click Chemistry for Constructing Fibrinolytic Cell Surfaces. [Abstract]2024 Jul 17;16(28):35874-35886. PMID: 38954798 -
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (232.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2.5 mg/mL (5.81 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Han SS, et, al. Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking. Theranostics. 2017 Mar 1;7(5):1164-1176. [Content Brief]
[2]. Han SS, et, al. Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking. Sci Rep. 2018 Sep 4;8(1):13212. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.3236 mL | 11.6179 mL | 23.2358 mL | 58.0896 mL |
| 5 mM | 0.4647 mL | 2.3236 mL | 4.6472 mL | 11.6179 mL | |
| DMSO | 10 mM | 0.2324 mL | 1.1618 mL | 2.3236 mL | 5.8090 mL |
| 15 mM | 0.1549 mL | 0.7745 mL | 1.5491 mL | 3.8726 mL | |
| 20 mM | 0.1162 mL | 0.5809 mL | 1.1618 mL | 2.9045 mL | |
| 25 mM | 0.0929 mL | 0.4647 mL | 0.9294 mL | 2.3236 mL | |
| 30 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
| 40 mM | 0.0581 mL | 0.2904 mL | 0.5809 mL | 1.4522 mL | |
| 50 mM | 0.0465 mL | 0.2324 mL | 0.4647 mL | 1.1618 mL | |
| 60 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9682 mL | |
| 80 mM | 0.0290 mL | 0.1452 mL | 0.2904 mL | 0.7261 mL | |
| 100 mM | 0.0232 mL | 0.1162 mL | 0.2324 mL | 0.5809 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ac4ManNAz (80% α isomer) Related Classifications
HY-118297 Related Classifications
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