Clofazimine

Name: Clofazimine
Brand: MedChemExpress
SKU: HY-B1046
Rating: 5.0 (12 reviews)

Cat. No.: HY-B1046 Purity: 99.79%: Data Sheet
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Clofazimine is an orally-active anti-mycobacterial agent with a wide range of anti-mycobacterial activity including leprosy and tuberculosis. Clofazimine exerts anti-inflammatory activities and anti-tumor activities by interfering DNA replication and inhibiting IL2 (IC₅₀ = 1.10 ± 0.26 μM, Jurkat T) production. Clofazimine can be used in mycobacterial and cancer research.

For research use only. We do not sell to patients.

CAS No. : 2030-63-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Clofazimine:

Clofazimine-d₇ In-stock
Clofazimine (Standard) Get quote

12 Publications Citing Use of MCE Clofazimine

RT-PCR

In Vivo Efficacy Study

Cell Proliferation/Viability Assay

Flow Cytometry

: Clofazimine purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Mar 21;11(12):eads9182. [Abstract]; Clofazimine (15 μM; 24 h) significantly blocked OA-induced expression of H3K4me3-controlled genes AGR2 and PARM1, with a trend toward reduced PHGDH and NTRK2 expression in microstructures from BRCA1 wild-type individuals.

: Clofazimine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 6;8(1):1425. [Abstract]; Clofazimine (CFZ) (3.125 μg/mL; 48 h) markedly reduced the survival of ΔcysE Mycobacterium tuberculosis H37Rv.

: Clofazimine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 6;8(1):1425. [Abstract]; Clofazimine (CFZ) (15.625-31.25 μg/mL; 1-3 h) strongly increased ROS levels in M. tb (Mycobacterium tuberculosis).

: Clofazimine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 6;8(1):1425. [Abstract]; Early-log-phase cultures of WT and ΔcysE M. tb (Mycobacterium tuberculosis) were treated with or without Clofazimine (CFZ) (3.125–6.25 μg/mL; 7 d). Survival was determined in the form of viable bacterial count expressed as CFU.

: Clofazimine purchased from MedChemExpress. Usage Cited in: Patent. US20250312336A1.; The weights of orthotopic syngeneic pancreatic tumors from C57BL/6J mice followed treatment with ANO6 inhibitors Clofazimine (50 mg/kg; p.o.; 3 weeks).

: Clofazimine purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Mar 12;8(1):111. [Abstract]; Clofazimine (Verteporfin) (5 μM) reduced the expression of steogenesis related genes (RUNX2, ALP, OSX) in BMSCs.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Quinolone

Cellular Effect

Cell Line	Type	Value	Description	References
BT-20	IC₅₀	19 μM Compound: Clofazimine	Cytotoxicity against human BT-20 cells incubated for 3 to 4 days by MTT assay Cytotoxicity against human BT-20 cells incubated for 3 to 4 days by MTT assay	[PMID: 34116325]
HMEC-1	CC₅₀	18.6 μM Compound: CFM	Cytotoxicity against human HMEC1 cells assessed as reduction in cell viability after 72 hrs by MTT method Cytotoxicity against human HMEC1 cells assessed as reduction in cell viability after 72 hrs by MTT method	[PMID: 25464882]
HMEC-1	IC₅₀	18.59 μM Compound: Clofazimine	Cytotoxicity against HMEC1 cells after 72 hrs by MTT assay Cytotoxicity against HMEC1 cells after 72 hrs by MTT assay	[PMID: 25497962]
HepG2	CC₅₀	49.02 μg/mL Compound: CLFZ	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 38784457]
Huh-7	CC₅₀	44.7 μM Compound: 1	Cytotoxicity against human HuH-7 cells assessed as reduction in cell viability incubated for 24 hrs by microplate reader assay Cytotoxicity against human HuH-7 cells assessed as reduction in cell viability incubated for 24 hrs by microplate reader assay	[PMID: 35279610]
MT4	CC₅₀	6.8 μM Compound: CFM	Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 96 hrs by MTT method Cytotoxicity against human MT4 cells assessed as reduction in cell viability after 96 hrs by MTT method	[PMID: 25464882]
NIH-3T3-G185	IC₅₀	0.6 μM Compound: Clofazimine	TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells	[PMID: 11716514]
NIH-3T3-G185	IC₅₀	1 μM Compound: Clofazimine	TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
NIH-3T3-G185	IC₅₀	1.1 μM Compound: Clofazimine	TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
Vero	IC₅₀	68.6 μg/mL Compound: CFZ	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 22931472]
Vero 76	CC₅₀	> 100 μM Compound: CFM	Cytotoxicity against african green monkey Vero 76 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT method Cytotoxicity against african green monkey Vero 76 cells assessed as reduction in cell viability after 48 to 96 hrs by MTT method	[PMID: 25464882]

In Vitro

Clofazimine (0.0625-2 mg/L, 14 d) exerts no apparent activity against M. tuberculosis during the first 2–4 days of exposure, exhibits a concentration-dependent antimicrobial activity after 1 week: bacteriostatic activity at concentrations at or below the 0.25 mg/L MIC and bactericidal activity at concentrations above the MIC. INH is used as positive control.^[3].
Clofazimine (10 μM, 24 h) inhibits the growth of hematological cancer cell lines (Jurkat, U266, Namalwa, K562, HL60)^[4].
Clofazimine (1-50 μM, 12-48 h) shows a dose- and time-dependent inhibitory effect for U266^[4].
Clofazimine (10 μM, 24-48 h) depolarizes the mitochondrial membrane, significantly increases active caspase-3 level (25-fold) and increases the percentage of early and late apoptotic cells in U266^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Clofazimine (100,50 mg/kg, p.o., once per day for 14 d) exhibits a delayed antimicrobial activity against Mycobacterium tuberculosis in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c mice (age 6–8 weeks, mass 18–20 g)^[3]
Dosage:	100, 50, 25, 12.5, 6.25, 3.125 or 1.5625 mg/kg
Administration:	Oral gavage (p.o.), once per day for 14 d
Result:	Exhibited an increase of the lung bacterial load during the first 7 days of treatment, and decreased the CFU counts in the lungs of mice after 10 days of treatment. Significantly declined the lung CFU counts in 100 or 50 mg/ kg group at 14th day.

Clinical Trial

Molecular Weight

473.40

Formula

C₂₇H₂₂Cl₂N₄

CAS No.

2030-63-9

Appearance

Solid

Color

Pink to red

SMILES

CC(/N=C1C(NC2=CC=C(Cl)C=C2)=CC3=NC4=C(C=CC=C4)N(C5=CC=C(Cl)C=C5)C3=C/1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL (13.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1124 mL	10.5619 mL	21.1238 mL
5 mM	0.4225 mL	2.1124 mL	4.2248 mL
10 mM	0.2112 mL	1.0562 mL	2.1124 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (279 KB) SDS (396 KB)

COA (251 KB) RP-HPLC (244 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Riccardi N, et al. Clofazimine: an old drug for never-ending diseases. Future Microbiol. 2020，15:557-566. [Content Brief]

[2]. Arbiser JL, et al. Clofazimine: a review of its medical uses and mechanisms of action. J Am Acad Dermatol. 1995 Feb;32(2 Pt 1):241-7. [Content Brief]

[3]. Ammerman NC, et al. Clofazimine has delayed antimicrobial activity against Mycobacterium tuberculosis both in vitro and in vivo. J Antimicrob Chemother. 2017，72(2):455-461. [Content Brief]

[4]. Durusu İZ, et al. Anti-cancer effect of clofazimine as a single agent and in combination with cisplatin on U266 multiple myeloma cell line. Leuk Res. 2017，55:33-40. [Content Brief]

[5]. Ren YR, et al. Clofazimine Inhibits Human Kv1.3 Potassium Channel by Perturbing Calcium Oscillation in T Lymphocytes. PLoS One. 2008，3(12):e4009. [Content Brief]

[6]. Cholo MC, et al. Clofazimine: current status and future prospects. J Antimicrob Chemother. 2012 Feb;67(2):290-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1124 mL	10.5619 mL	21.1238 mL	52.8095 mL
	5 mM	0.4225 mL	2.1124 mL	4.2248 mL	10.5619 mL
	10 mM	0.2112 mL	1.0562 mL	2.1124 mL	5.2809 mL

Clofazimine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Clofazimine

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12 Publications Citing Use of MCE Clofazimine

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Clofazimine Related Antibodies

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Complete Stock Solution Preparation Table

Clofazimine Related Classifications

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