Daidzein
Based on 26 publication(s) in Google Scholar
Daidzein is a soy isoflavone, which acts as a PPAR activator.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 486-66-8
- Formula: C15H10O4
- Molecular Weight:254.24
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)
Publications Citing Use of MedChemExpress (MCE) Daidzein
More- Cell. 2025 May 29;188(11):3065-3080.e21. [Abstract]
- Microbiome. 2025 Jun 18;13(1):145. [Abstract]
- Sci Adv. 2025 Jan 10;11(2):eadt0925. [Abstract]
- Cell Rep Med. 2022 May 17;3(5):100608. [Abstract]
- Food Chem. 2026 Jul 30:518:149561. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 May 31:489:144992. [Abstract]
- Int J Biol Macromol. 2025 Jan 24:140314. [Abstract]
- Phytomedicine. 2025 Dec:149:157488. [Abstract]
- Phytomedicine. 2025 May:140:156484. [Abstract]
- Food Front. 2026 Feb 16;7(2):e70247.
- J Agric Food Chem. 2026 Mar 4;74(8):7125-7138. [Abstract]
- Food Funct. 2025 Jun 16;16(12):4822-4836. [Abstract]
- J Ethnopharmacol. 2024 Oct 23:118972. [Abstract]
- J Ethnopharmacol. 2024 May 10:325:117824. [Abstract]
- Microb Biotechnol. 2026 Jun;19(6):e70366. [Abstract]
- Microchem J. 2020, 104771.
- Eur J Pharmacol. 2022 Mar 15;919:174805. [Abstract]
- Eur J Pharmacol. 2020 Oct 15;885:173399. [Abstract]
- Mol Neurobiol. 2025 Apr;62(4):4274-4291. [Abstract]
- Mol Neurobiol. 2024 Jul;61(7):4166-4177. [Abstract]
- Genomics. 2021 Jul;113(4):2702-2716. [Abstract]
- PeerJ. 2023 Oct 16:11:e16121. [Abstract]
- Planta Med. 2021 Jun;87(7):538-549. [Abstract]
- SSRN. 2025 Sep 8.
- bioRxiv. 2025 Jan 03.
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
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Cell Imaging/Staining
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In Vivo Efficacy Study
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WB
All Endogenous Metabolite Isoforms
More
Biological Activity
|
PPAR-α |
PPAR-γ |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
>100 μg/mL
Compound: 8
|
Inhibition of EGFR in human A431 cells
Inhibition of EGFR in human A431 cells
|
[PMID: 1479375] |
| A549 | IC50 |
>100 μM
Compound: 12
|
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| B16-BL6 | IC50 |
>100 μM
Compound: 12
|
Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| BV-2 | IC50 |
>50 μM
Compound: 8
|
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production
|
[PMID: 29482940] |
| HEK293 | IC50 |
120 μM
Compound: Daidzein
|
Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method
Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method
|
[PMID: 21030469] |
| HEK293 | IC50 |
7.3 μM
Compound: Daidzein
|
Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method
Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method
|
[PMID: 21030469] |
| HeLa | IC50 |
>100 μM
Compound: 12
|
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| HepG2 | IC50 |
>100 μM
Compound: Daidzein
|
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
|
[PMID: 26896708] |
| HT-1080 | IC50 |
>100 μM
Compound: 12
|
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| Ishikawa | IC50 |
1.2 μM
Compound: daidzein
|
Estrogenic activity in human Ishikawa cells assessed as induction of alkaline phosphatase activity after 4 days by para-nitrophenol release assay
Estrogenic activity in human Ishikawa cells assessed as induction of alkaline phosphatase activity after 4 days by para-nitrophenol release assay
|
[PMID: 12502307] |
| L02 | IC50 |
>100 μM
Compound: Daidzein
|
Cytotoxicity against human LO2 cells after 48 hrs by MTT assay
Cytotoxicity against human LO2 cells after 48 hrs by MTT assay
|
[PMID: 26896708] |
| Lewis lung carcinoma cell line | IC50 |
>100 μM
Compound: 12
|
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| MCF7 | GI50 |
122 μM
Compound: 1
|
Antiproliferative activity against estrogen receptor expressing human MCF7 cells
Antiproliferative activity against estrogen receptor expressing human MCF7 cells
|
[PMID: 19818612] |
| MCF7 | IC50 |
10 nM
Compound: Daidzein
|
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay
|
[PMID: 33257172] |
| MDA-MB-436 | GI50 |
>196 μM
Compound: 1
|
Antiproliferative activity against estrogen receptor expressing human MDA-MB-436 cells
Antiproliferative activity against estrogen receptor expressing human MDA-MB-436 cells
|
[PMID: 19818612] |
| MDCK | CC50 |
>787 μM
Compound: 18
|
Cytotoxicity against MDCK cells by MTT assay
Cytotoxicity against MDCK cells by MTT assay
|
[PMID: 18640042] |
| Melan-a | IC50 |
>500 μM
Compound: 4
|
Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA
Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA
|
[PMID: 20022495] |
| Neutrophil | IC50 |
24.22 μg/mL
Compound: 17
|
Anti-inflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
Anti-inflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced elastase release
|
[PMID: 21848266] |
| Neutrophil | IC50 |
6.19 μg/mL
Compound: 17
|
Anti-inflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced superoxide anion generation
Anti-inflammatory activity in human neutrophils assessed as inhibition of FMLP/CB-induced superoxide anion generation
|
[PMID: 21848266] |
| RAW264.7 | IC50 |
>100 μM
Compound: 5
|
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
|
[PMID: 23743282] |
| Ventricular myocyte | IC50 |
12 μM
Compound: Dzaidzein
|
Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes
Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes
|
[PMID: 22761000] |
In 3T3-L1 adipocytes, Daidzein inverses the attenuation of adiponectin gene expression by co-culture, and these effects are inhibited by the PPAR-γ specific inhibitor. Daidzein attenuates the reduction of adiponectin expression in adipocytes, and a PPAR-γ specific inhibitor abrogated this effect. Direct activation of PPAR-α and-γ by Daidzein is confirmed by a luciferase reporter assay. In HEK293T cells, Daidzein significantly increases PPAR-α transcriptional activity in a concentration-dependent manner. Although an obvious dose-dependency is not observed in PPAR-γ transcriptional activity, Daidzein also significantly increases PPAR-γ transcriptional activity over a similar range of concentrations at which Daidzein enhanced PPAR-α transcriptional activity, with a maximum increase at 25 μM[1]. Daidzein is a soy isoflavone, which upregulates the expression of Abcg1, and it promotes axonal outgrowth in cultured hippocampal neurons via estrogen receptor signaling. Daidzein is a major component of soy with structural similarity to estrogen. It exerts an anti-inflammatory effect, lowers lipid levels, and increases mitochondrial biogenesis. As an activator of nuclear receptor peroxisome proliferator-activated receptors (PPARs), Daidzein enhances transcription of PPARs-dependent genes, including liver X receptors (LXRs, Nr1h gene family in mice). Incubation with different concentrations of Daidzein, from 5 to 100 μM, increases APOE transcriptional activity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 486-66-8
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Appearance Solid
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Molecular Weight 254.24
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Formula C15H10O4
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Color White to off-white
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SMILES
O=C1C(C2=CC=C(O)C=C2)=COC3=CC(O)=CC=C13
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)
Publications (26)
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Journal Impact Factor
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Most Recent
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Cell
Microbiome metabolism of dietary phytochemicals controls the anticancer activity of PI3K inhibitors. [Abstract]2025 May 29;188(11):3065-3080.e21. PMID: 40393457 -
Microbiome
Maternal intestinal L. vaginalis facilitates embryo implantation and survival through enhancing uterine receptivity in sows. [Abstract]2025 Jun 18;13(1):145. PMID: 40533850
Daidzein purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Jun 18;13(1):145. [Abstract]
Daidzein could markedly motivate the adhesion of PTCs to endometrial epithelium by fluorescence microscopy.
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Sci Adv
2025 Jan 10;11(2):eadt0925. PMID: 39772695 -
Cell Rep Med
Management of prostate cancer by targeting 3βHSD1 after enzalutamide and abiraterone treatment. [Abstract]2022 May 17;3(5):100608. PMID: 35584629
Daidzein purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2022 May 17;3(5):100608. [Abstract]
Daidzein regulated Abi metabolism in VCaP cells. Abi, 10 nM; BCA and its derivatives, 1 μM.
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Food Chem
Distribution profiles of 12 isoflavone monomers in Chinese soybeans from saline-alkali and ordinary soils and subsequent supercritical CO₂ extraction optimization. [Abstract]2026 Jul 30:518:149561. PMID: 42114489 -
Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Int J Biol Macromol
Mechanism of dsDNA binding, enzyme inhibition, antioxidant activities, and molecular docking studies of taxifolin, daidzein, and S-equol. [Abstract]2025 Jan 24:140314. PMID: 39864700 -
Phytomedicine
Daidzein reprograms EP300/CREBBP-deficient immune evasion via targeting the PPARγ-ANGPT4/Tie2 axis in hypopharyngeal squamous cell carcinoma. [Abstract]2025 Dec:149:157488. PMID: 41240538
Daidzein purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157488. [Abstract]
Transwell assays show that Daidzein (20-40 µM) significantly inhibits invasion.
Daidzein purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157488. [Abstract]
TUNEL assays show that Daidzein (20-40 µM) significantly promotes apoptosis.
Daidzein purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157488. [Abstract]
In vivo xenograft tumor model. Nude mice injected with HPSCC cells and treated with Daidzein (20-40 mg/kg, i.p.) exhibited significantly reduced tumor volume and weight compared with vehicle controls, confirming its anti-tumor efficacy in vivo.
Daidzein purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Dec:149:157488. [Abstract]
Western blot analysis showing that Daidzein (20, 40 μM) treatment increased PPARγ expression while reducing ANGPT4 and Tie2 protein levels, indicating restoration of the tumor-suppressive signaling axis.
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Phytomedicine
Fangchinoline suppresses nasopharyngeal carcinoma progression by inhibiting SQLE to regulate the PI3K/AKT pathway dysregulation. [Abstract]2025 May:140:156484. PMID: 40090046 -
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J Agric Food Chem
Daidzein Confers Prophylactic Protection against Food Allergy by Restoring Immune Regulation, Intestinal Barrier Integrity, and Microbiota-Metabolite Homeostasis. [Abstract]2026 Mar 4;74(8):7125-7138. PMID: 41705388 -
Food Funct
Daidzein alleviates CCl4- and BDL-induced liver fibrosis via suppressing the integrin alphaVbeta1/YAP signaling. [Abstract]2025 Jun 16;16(12):4822-4836. PMID: 40407304 -
J Ethnopharmacol
Desmodium styracifolium (Osb.) Merr. Extracts alleviate cholestatic liver disease by FXR pathway. [Abstract]2024 Oct 23:118972. PMID: 39454708 -
J Ethnopharmacol
Cornus officinalis var. koreana Kitam extracts alleviate cadmium-induced renal fibrosis by targeting matrix metallopeptidase 9. [Abstract]2024 May 10:325:117824. PMID: 38278375 -
Microb Biotechnol
The Flavonoids Daidzein and Genistein Induce Wall-Deficient Cell Formation in Streptomyces coelicolor Under Hyperosmotic Stress. [Abstract]2026 Jun;19(6):e70366. PMID: 42246457 -
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Eur J Pharmacol
Daidzein suppresses TGF-β1-induced cardiac fibroblast activation via the TGF-β1/SMAD2/3 signaling pathway. [Abstract]2022 Mar 15;919:174805. PMID: 35151651 -
Eur J Pharmacol
Daidzein ameliorates LPS-induced hepatocyte injury by inhibiting inflammation and oxidative stress. [Abstract]2020 Oct 15;885:173399. PMID: 32712091 -
Mol Neurobiol
Dietary Flavonoid Chrysin Functions as a Dual Modulator to Attenuate Amyloid-β and Tau Pathology in the Models of Alzheimer's Disease. [Abstract]2025 Apr;62(4):4274-4291. PMID: 39432184 -
Mol Neurobiol
Inhibiting Caveolin-1-Related Akt/mTOR Signaling Pathway Protects Against N-methyl-D-Aspartate Receptor Activation-Mediated Dysfunction of Blood-Brain Barrier in vitro. [Abstract]2024 Jul;61(7):4166-4177. PMID: 38066401 -
Genomics
Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides. [Abstract]2021 Jul;113(4):2702-2716. PMID: 34111523 -
PeerJ
2023 Oct 16:11:e16121. PMID: 37868048 -
Planta Med
Compound Cocktail Inhibits Influenza Viral Pneumonia via Phospholipase Cγ1 Phosphorylation-Related Necroptosis and Partial Autophagy in Natural Killer Cells. [Abstract]2021 Jun;87(7):538-549. PMID: 33545719 -
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Solvent & Solubility
DMSO : ≥ 50 mg/mL (196.66 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 20 mg/mL (78.67 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
HEK293T cells are plated on 24-well plates at a cell density of approximately 2.5×104 cells/well and are grown to 70-80% confluence. Cells are then transiently transfected with a PPAR-α or PPAR-γ expression plasmid, and a plasmid containing the luciferase gene under the control of three tandem PPAR response elements (PPRE × 3 TK-luciferase) using an X-treme GENE HP DNA Transfection Reagent. Renilla luciferase control vectors are co-transfected to control for transfection efficiency. After transfection, cells are cultured for another 24 h in medium containing DMSO or various concentrations (6.25, 12.5, 25 μM) of Daidzein. Cells are lysed, and luciferase activity is measured and expressed as fold induction, that is normalized to the activity of the renilla luciferase control plasmid[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
Experiments are performed in 10- to 11-week-old male C57 (C57 bl/6) and Apoe KO (C57 background) mice. For long-term stroke recovery, mice receive Moxifloxacin (100 mg/kg) for 3 d. The prophylactic antibiotic treatment is shown to effectively reduce mortality in an animal model of stroke by attenuating peripheral infection. In addition, saline is subcutaneously administered daily, and hydrogel (Clear H2O) is given to prevent dehydration. With the implementation of poststroke care (antibiotic regimen, rehydration, and feeding hydrogels with soft diet) during the acute period (<1 week), mice start to regain their body weight by day 5 and continue to recover from stroke. Animals are randomly selected for vehicle or Daidzein treatment. Vehicle or Daidzein (10 mg/kg) is administered subcutaneously within 30 min of reperfusion after confirming the reperfusion of blood flow, daily for 7 d and then every other day up to 1 month.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sakamoto Y1, et al. The Dietary Isoflavone Daidzein Reduces Expression of Pro-Inflammatory Genes through PPARα/γ and JNK Pathways in Adipocyte and Macrophage Co-Cultures. PLoS One. 2016 Feb 22;11(2):e0149676. [Content Brief]
[2]. Kim E, et al. Daidzein Augments Cholesterol Homeostasis via ApoE to Promote Functional Recovery in Chronic Stroke. J Neurosci. 2015 Nov 11;35(45):15113-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9333 mL | 19.6664 mL | 39.3328 mL | 98.3319 mL |
| 5 mM | 0.7867 mL | 3.9333 mL | 7.8666 mL | 19.6664 mL | |
| 10 mM | 0.3933 mL | 1.9666 mL | 3.9333 mL | 9.8332 mL | |
| 15 mM | 0.2622 mL | 1.3111 mL | 2.6222 mL | 6.5555 mL | |
| 20 mM | 0.1967 mL | 0.9833 mL | 1.9666 mL | 4.9166 mL | |
| 25 mM | 0.1573 mL | 0.7867 mL | 1.5733 mL | 3.9333 mL | |
| 30 mM | 0.1311 mL | 0.6555 mL | 1.3111 mL | 3.2777 mL | |
| 40 mM | 0.0983 mL | 0.4917 mL | 0.9833 mL | 2.4583 mL | |
| 50 mM | 0.0787 mL | 0.3933 mL | 0.7867 mL | 1.9666 mL | |
| 60 mM | 0.0656 mL | 0.3278 mL | 0.6555 mL | 1.6389 mL | |
| 80 mM | 0.0492 mL | 0.2458 mL | 0.4917 mL | 1.2291 mL | |
| 100 mM | 0.0393 mL | 0.1967 mL | 0.3933 mL | 0.9833 mL |