Duvelisib
Based on 17 publication(s) in Google Scholar
Duvelisib (IPI-145) is a selectivite p100δ inhibitor with IC50 of 2.5 nM, 27.4 nM, 85 nM and 1602 nM for p110δ, P110γ, p110β and p110α, respectively.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 1201438-56-3
- Formula: C22H17ClN6O
- Molecular Weight:416.86
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) Duvelisib
More- Nat Metab. 2020 Dec;2(12):1427-1442. [Abstract]
- Nat Commun. 2020 Apr 14;11(1):1792. [Abstract]
- Sci Transl Med. 2018 Jul 18;10(450):eaaq1093. [Abstract]
- Phytomedicine. 2025 May 27:143:156911. [Abstract]
- Cell Syst. 2018 Apr 25;6(4):424-443.e7. [Abstract]
- Blood Adv. 2025 Jul 2:bloodadvances.2024015322. [Abstract]
- Oncoimmunology. 2022 Oct 11;11(1):2134536. [Abstract]
- Biochem Pharmacol. 2026 Mar 28;250(Pt 1):117939. [Abstract]
- Arab J Chem. 2023 Jul 4, 105117.
- RSC Adv. 2025 Dec 18;15(59):50944-50962. [Abstract]
- Eur J Immunol. 2025 Oct;55(10):e70068. [Abstract]
- Am J Cancer Res. 2025 May 15;15(5):2097-2110. [Abstract]
- Anal Methods. 2026 Feb 5;18(5):1101-1115. [Abstract]
- University of California. 2025.
- bioRxiv. 2025 May 13:2024.10.01.616161. [Abstract]
- Res Sq. 2024 Jun 13.
- bioRxiv. 2024 Mar 28:2023.05.15.540704. [Abstract]
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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WB
Biological Activity
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p110δ 2.5 nM (IC50) |
p110γ 27.4 nM (IC50) |
p110β 85 nM (IC50) |
p110α 1602 nM (IC50) |
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Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 4T1 | IC50 |
22.88 μM
Compound: Duvelisib
|
Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| A549 | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| B16-F10 | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| DOHH-2 | IC50 |
0.2 μM
Compound: 1; IPI-145
|
Antiproliferative activity against human DOHH-2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Antiproliferative activity against human DOHH-2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35831917] |
| HepG2 | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| Huh-7 | IC50 |
6.57 μM
Compound: Duvelisib
|
Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| Jurkat | IC50 |
1.9 μM
Compound: Duvelisib
|
Antiproliferative activity against human Jurkat cells after 3 days by CellTiter-Glo assay
Antiproliferative activity against human Jurkat cells after 3 days by CellTiter-Glo assay
|
[PMID: 27774127] |
| Jurkat | IC50 |
3.17 μM
Compound: Duvelisib
|
Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| L929 | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against mouse L929 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against mouse L929 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| LOUCY | IC50 |
0.98 μM
Compound: Duvelisib
|
Antiproliferative activity against human Loucy cells after 3 days by CellTiter-Glo assay
Antiproliferative activity against human Loucy cells after 3 days by CellTiter-Glo assay
|
[PMID: 27774127] |
| MCF7 | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| MDA-MB-231 | IC50 |
9.76 μM
Compound: Duvelisib
|
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| MOLM-14 | IC50 |
1.2 μM
Compound: Duvelisib
|
Antiproliferative activity against human MOLM14 cells after 3 days by CellTiter-Glo assay
Antiproliferative activity against human MOLM14 cells after 3 days by CellTiter-Glo assay
|
[PMID: 27774127] |
| MOLT-4 | IC50 |
2.3 μM
Compound: Duvelisib
|
Antiproliferative activity against human MOLT4 cells after 3 days by CellTiter-Glo assay
Antiproliferative activity against human MOLT4 cells after 3 days by CellTiter-Glo assay
|
[PMID: 27774127] |
| MV4-11 | IC50 |
4.4 μM
Compound: Duvelisib
|
Antiproliferative activity against human MV4-11 cells after 3 days by CellTiter-Glo assay
Antiproliferative activity against human MV4-11 cells after 3 days by CellTiter-Glo assay
|
[PMID: 27774127] |
| Raji | IC50 |
0.36 nM
Compound: 15
|
Cytotoxicity against in human Raji cells
Cytotoxicity against in human Raji cells
|
[PMID: 30582813] |
| Raji | IC50 |
2.5 nM
Compound: 15
|
Inhibition of PI3Kdelta in human Raji cells
Inhibition of PI3Kdelta in human Raji cells
|
[PMID: 30582813] |
| Raji | IC50 |
27 nM
Compound: 15
|
Inhibition of PI3Kgamma in human Raji cells
Inhibition of PI3Kgamma in human Raji cells
|
[PMID: 30582813] |
| Ramos | IC50 |
0.001 μM
Compound: 4; IPI-145
|
Inhibition of PI3Kdelta in human Ramos cells assessed as reduction in antihuman IgM-stimulated AKT phosphorylation at Ser473 residue preincubated for 60 mins followed by antihuman IgM stimulation and measured after 15 mins
Inhibition of PI3Kdelta in human Ramos cells assessed as reduction in antihuman IgM-stimulated AKT phosphorylation at Ser473 residue preincubated for 60 mins followed by antihuman IgM stimulation and measured after 15 mins
|
[PMID: 31033293] |
| RAW264.7 | IC50 |
0.025 μM
Compound: 4; IPI-145
|
Inhibition of PI3Kgamma in rat RAW264.7 cells assessed as reduction in C5a-stimulated AKT phosphorylation at Ser473 residue preincubated for 30 mins followed by C5a-stimulation and measured after 5 mins
Inhibition of PI3Kgamma in rat RAW264.7 cells assessed as reduction in C5a-stimulated AKT phosphorylation at Ser473 residue preincubated for 30 mins followed by C5a-stimulation and measured after 5 mins
|
[PMID: 31033293] |
| RAW264.7 | IC50 |
13.2 μM
Compound: Duvelisib
|
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| Sf21 | IC50 |
0.001 μM
Compound: 4; IPI-145
|
Inhibition of N-terminal His6-tagged recombinant full length human p110delta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence po
Inhibition of N-terminal His6-tagged recombinant full length human p110delta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence po
|
[PMID: 31033293] |
| Sf21 | IC50 |
0.008 μM
Compound: 4; IPI-145
|
Inhibition of N-terminal His6-tagged recombinant full length human p110beta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence pol
Inhibition of N-terminal His6-tagged recombinant full length human p110beta/untagged recombinant full length human p85alpha expressed in baculovirus infected Sf21 insect cells using PIP2 as substrate measured after 80 mins by transcreener fluorescence pol
|
[PMID: 31033293] |
| SUD4 | IC50 |
0.27 μM
Compound: 1; IPI-145
|
Antiproliferative activity against human SU-DHL-4 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Antiproliferative activity against human SU-DHL-4 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35831917] |
| SU-DHL-6 | IC50 |
0.39 μM
Compound: 1; IPI-145
|
Antiproliferative activity against human SU-DHL-6 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Antiproliferative activity against human SU-DHL-6 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35831917] |
| SU-DHL-6 | IC50 |
0.4 nM
Compound: 1; IPI-145
|
Antiproliferative activity against human SUDHL2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Antiproliferative activity against human SUDHL2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35831917] |
| T47D | IC50 |
>100 μM
Compound: Duvelisib
|
Cytotoxicity against human T47D cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human T47D cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
| THP-1 | IC50 |
14.89 μM
Compound: Duvelisib
|
Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
|
[PMID: 37011446] |
PI3Kδ and PI3Kγ inhibition with Duvelisib (IPI-145) has anti-proliferative activity in primary AML cells by inhibiting the activity of AKT and MAPK. Pre-treatment of AML cells with Duvelisib inhibits both adhesion and migration of AML blasts to bone marrow stromal cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1201438-56-3
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Appearance Solid
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Molecular Weight 416.86
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Formula C22H17ClN6O
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Color Off-white to gray
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SMILES
O=C1N(C2=CC=CC=C2)C([C@@H](NC3=C4N=CNC4=NC=N3)C)=CC5=C1C(Cl)=CC=C5
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Synonyms
IPI-145; INK1197
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (17)
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Journal Impact Factor
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Most Recent
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Nat Metab
The PI3K pathway preserves metabolic health through MARCO-dependent lipid uptake by adipose tissue macrophages. [Abstract]2020 Dec;2(12):1427-1442. PMID: 33199895 -
Nat Commun
Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. [Abstract]2020 Apr 14;11(1):1792. PMID: 32286289 -
Sci Transl Med
PP2A inhibition is a druggable MEK inhibitor resistance mechanism in KRAS-mutant lung cancer cells. [Abstract]2018 Jul 18;10(450):eaaq1093. PMID: 30021885 -
Phytomedicine
CBX5 loss drives Pl3Kδ inhibitor resistance in mantle cell lymphoma and propolis restores sensitivity by inducing CBX5-mediated ferroptosis. [Abstract]2025 May 27:143:156911. PMID: 40466505 -
Cell Syst
A Library of Phosphoproteomic and Chromatin Signatures for Characterizing Cellular Responses to Drug Perturbations. [Abstract]2018 Apr 25;6(4):424-443.e7. PMID: 29655704 -
Blood Adv
BH3 mimetic drugs overcome the microenvironment-induced resistance to crizotinib in ALK+ anaplastic large cell lymphoma. [Abstract]2025 Jul 2:bloodadvances.2024015322. PMID: 40601898
Duvelisib purchased from MedChemExpress. Usage Cited in: Blood Adv. 2025 Jul 2:bloodadvances.2024015322. [Abstract]
A bar plot reported the percent viability assessed by propidium iodide (PI) incorporation in TLBR2 cells cultured alone or in the presence of MS-5 and treated with navitoclax (1-2 µM) for 72 hours.
Duvelisib purchased from MedChemExpress. Usage Cited in: Blood Adv. 2025 Jul 2:bloodadvances.2024015322. [Abstract]
Heat map reported cell viability of the ALK+ 36 ALCL SUPM2 cell line treated for 72 hours with the indicated drugs (x-axis) and concentrations (y-axis).
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Oncoimmunology
RHOA G17V induces T follicular helper cell specification and involves angioimmunoblastic T-cell lymphoma via upregulating the expression of PON2 through an NF-κB-dependent mechanism. [Abstract]2022 Oct 11;11(1):2134536. PMID: 36249275
Duvelisib purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2022 Oct 11;11(1):2134536. [Abstract]
Immunoblots showed that Duvelisib (24 h) suppressed the expression of Pon2, decreased the phosphorylated IκB kinase (IKK) complex, and activated the NF-κB p65 in Rhoa G17V-expressing CD4+ T cells.
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Biochem Pharmacol
Duvelisib upregulates p27 expression and leads to intestinal damage via the NEDD4L/CK1ε axis. [Abstract]2026 Mar 28;250(Pt 1):117939. PMID: 41905427 -
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RSC Adv
Integrative machine learning-guided in silico and in vitro approach reveals selective small molecule inhibitors targeting mutant IDH1. [Abstract]2025 Dec 18;15(59):50944-50962. PMID: 41426059
Duvelisib purchased from MedChemExpress. Usage Cited in: RSC Adv. 2025 Dec 18;15(59):50944-50962. [Abstract]
RMSD plots showed structural dynamics upon binding with the inhibitors during a 250 ns MD simulation ligand, for standard mutant IDH1 inhibitors, olutasidenib (black) and α-mangostin (red), screened inhibitors, atogepant (green), dacomitinib (blue), duvelisib (yellow), idelalisib (brown), and vandetanib (turquoise), and an inactive reference compound, temozolomide (orange).
Duvelisib purchased from MedChemExpress. Usage Cited in: RSC Adv. 2025 Dec 18;15(59):50944-50962. [Abstract]
Hbond analysis was performed during the 250 ns MD simulation for the standard mutant IDH1 inhibitor duvelisib. Each horizontal line on the y-axis represented the number of hydrogen bonds formed simultaneously, with its duration shown along the time on the (x) axis. A higher and denser clustering of lines suggested stronger and more stable interactions throughout the simulation period.
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Eur J Immunol
2025 Oct;55(10):e70068. PMID: 41054024 -
Am J Cancer Res
A long-lasting PI3Kδ inhibitor zandelisib forms a water-shielded hydrogen bond with p110δ and demonstrates sustained inhibitory effects. [Abstract]2025 May 15;15(5):2097-2110. PMID: 40520883 -
Anal Methods
Estimation of the metabolic stability of omipalisib in human liver microsomes employing an ultra-fast UPLC-MS/MS approach: in silico screening for structural alarms and metabolic lability. [Abstract]2026 Feb 5;18(5):1101-1115. PMID: 41586454 -
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bioRxiv
2025 May 13:2024.10.01.616161. PMID: 40236007 -
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bioRxiv
2024 Mar 28:2023.05.15.540704. PMID: 37292824
Solvent & Solubility
DMSO : 100 mg/mL (239.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.99 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Pillinger G, et al. Targeting PI3Kδ and PI3Kγ signalling disrupts human AML survival and bone marrow stromal cell mediated protection. Oncotarget. 2016 Jun 28;7(26):39784-39795. [Content Brief]
[2]. G?ckeritz E, et al. Efficacy of phosphatidylinositol-3 kinase inhibitors with diverse isoform selectivity profiles for inhibiting the survival of chronic lymphocytic leukemia cells. Int J Cancer. 2015 Nov 1;137(9):2234-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3989 mL | 11.9944 mL | 23.9889 mL | 59.9722 mL |
| 5 mM | 0.4798 mL | 2.3989 mL | 4.7978 mL | 11.9944 mL | |
| 10 mM | 0.2399 mL | 1.1994 mL | 2.3989 mL | 5.9972 mL | |
| 15 mM | 0.1599 mL | 0.7996 mL | 1.5993 mL | 3.9981 mL | |
| 20 mM | 0.1199 mL | 0.5997 mL | 1.1994 mL | 2.9986 mL | |
| 25 mM | 0.0960 mL | 0.4798 mL | 0.9596 mL | 2.3989 mL | |
| 30 mM | 0.0800 mL | 0.3998 mL | 0.7996 mL | 1.9991 mL | |
| 40 mM | 0.0600 mL | 0.2999 mL | 0.5997 mL | 1.4993 mL | |
| 50 mM | 0.0480 mL | 0.2399 mL | 0.4798 mL | 1.1994 mL | |
| 60 mM | 0.0400 mL | 0.1999 mL | 0.3998 mL | 0.9995 mL | |
| 80 mM | 0.0300 mL | 0.1499 mL | 0.2999 mL | 0.7497 mL | |
| 100 mM | 0.0240 mL | 0.1199 mL | 0.2399 mL | 0.5997 mL |