2. Cell Cycle/DNA Damage
    Epigenetics
  3. HDAC
  4. LMK-235

LMK-235 

Cat. No.: HY-18998 Purity: 98.97%
COA Handling Instructions

LMK-235 is a potent and selective HDAC4/5 inhibitor, inhibits HDAC5, HDAC4, HDAC6, HDAC1, HDAC2, HDAC11 and HDAC8, with IC50s of 4.22 nM, 11.9 nM, 55.7 nM, 320 nM, 881 nM, 852 nM and 1278 nM, respectively, and is used in cancer research.

For research use only. We do not sell to patients.

LMK-235 Chemical Structure

LMK-235 Chemical Structure

CAS No. : 1418033-25-6

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 66 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 96 In-stock
50 mg USD 300 In-stock
100 mg USD 540 In-stock
200 mg USD 960 In-stock
500 mg   Get quote  
1 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 18 publication(s) in Google Scholar

Top Publications Citing Use of Products

    LMK-235 purchased from MCE. Usage Cited in: Cancer Biol Ther. 2018;19(9):825-834.  [Abstract]

    OCILY10 cells are treated with different concentration of LMK-235 (0.5, 1.0, 1.5, 2.0 and 2.5 μM) for 24 hours,24 hours, 36 hours and 48 hours, and HDAC4 and HDAC5 protein levels are examined by Western blot .All experiments are conducted three times.

    LMK-235 purchased from MCE. Usage Cited in: Cancer Biol Ther. 2018;19(9):825-834.  [Abstract]

    OCI-LY3 cells are treated with different concentration of LMK-235 (0.5, 1.0, 1.5, 2.0 and 2.5 μM) for 24 hours,24 hours, 36 hours and 48 hours, and HDAC4 and HDAC5 protein levels are examined by Western blot .All experiments are conducted three times.

    LMK-235 purchased from MCE. Usage Cited in: Life Sci. 2018 Aug 15;207:386-394.  [Abstract]

    The protein levels of HDAC4, HDAC5, HO-1, histone 3 acetylation, histone 4 acetylation and Smad7 in CCRF-SB cells are detected by Western blot after LMK-235 treatment at 0.25, 0.5, 1, 2 and 4 μM for 24 h. β-Actin is used as internal reference.

    View All HDAC Isoform Specific Products:

    View All Isoforms
    HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    LMK-235 is a potent and selective HDAC4/5 inhibitor, inhibits HDAC5, HDAC4, HDAC6, HDAC1, HDAC2, HDAC11 and HDAC8, with IC50s of 4.22 nM, 11.9 nM, 55.7 nM, 320 nM, 881 nM, 852 nM and 1278 nM, respectively, and is used in cancer research.

    IC50 & Target

    IC50: 4.22 nM (HDAC5), 11.9 nM (HDAC4), 55.7 nM (HDAC6), 320 nM (HDAC1), 881 nM (HDAC2), 852 nM (HDAC11), 1278 nM (HDAC8)[1]
    In Vitro

    LMK-235 shows cytotoxic activity against human ovarian cancer cell lines A2780 and A2780 CisR, with IC50s of 0.49 μM and 0.32 μM, respectively. LMK-235 inhibits HDAC in A2780 and A2780 CisR cell lines, with IC50s of 0.65 μM and 0.32 μM, respectively. LMK-235 produces a higher reduction in cell viability in comparison to the combination of cisplatin and vorinostat in all cell lines[1]. LMK-235 (0, 0.625, 1.25, 2.5, 5, 10, and 20 μM) reduces the proliferation of BC cells in a dose- and time-dependent manner. LMK-235 (0-800 nM) also inhibits the growth of BC cells. Moreover, LMK-235 synergizes with bortezomib in BC cell lines[2]. LMK235 (2, 20 nM) decreases in HDAC4 nuclear accumulation in Cdkl5 -/Y NPCs, completely restores the reduced number of neurons generated from Cdkl5 -/Y NPCs. LMK235 also restores histone 3 acetylation in Cdkl5 -/Y NPCs. LMK235 causes a notable increase in the isoform IV, but does not affect BDNF isoforms I or II[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    In Vivo

    LMK235 (5 and 20 mg/kg) restores survival and maturation of postmitotic granule neurons in Cdkl5 -/Y mice. LMK235 also restores synapse development in the dentate gyrus and hippocampus of Cdkl5 -/Y mice. Furthermore, LMK235 restores hippocampus-dependent learning and memory in Cdkl5 -/Y mice[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    294.35

    Appearance

    Solid

    Formula

    C15H22N2O4
    CAS No.
    SMILES

    O=C(NOCCCCCC(NO)=O)C1=CC(C)=CC(C)=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (101.92 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3973 mL 16.9866 mL 33.9732 mL
    5 mM 0.6795 mL 3.3973 mL 6.7946 mL
    10 mM 0.3397 mL 1.6987 mL 3.3973 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (7.07 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (7.07 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (7.07 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.61%

    COA (242 KB) LCMS (269 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance is measured at 544 and 690 nm in a FLUOstar microplate reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    LMK-2351418033-25-6LMK235LMK 235HDACHistone deacetylasesInhibitorinhibitorinhibit

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