Anti-Mouse CD4 Antibody (GK1.5)
Based on 1 Customer Validation
Anti-Mouse CD4 Antibody (GK1.5) is an anti-mouse CD4 IgG2b monoclonal antibody. Anti-Mouse CD4 Antibody (GK1.5) can significantly deplete CD4+ T cells. Anti-Mouse CD4 Antibody (GK1.5) can extend the survival rate of xenograft models. Anti-Mouse CD4 Antibody (GK1.5) can be used for research on immunology. The recommend isotype control of Anti-Mouse CD4 Antibody (GK1.5): Rat IgG2b kappa, Isotype Control (HY-P990682).
For research use only. We do not sell to patients.
- Purity: 99%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG2b kappa
Mouse
CD4
Anti-Mouse CD4 Antibody (GK1.5) (on day 4 and 10) can be used to construct a vitiligo mouse model in C57BL/6J mice injected with 2×105 B16F10 melanoma cells[2].
Anti-Mouse CD4 Antibody (GK1.5) (5 mg/kg, i.p., on day -8 and 6) inhibits tumor metastasis and alters T cell count combined with the OVA peptide vaccine in C57BL/6 mice bearing EG7 tumors[3].
Anti-Mouse CD4 Antibody (GK1.5) (0.2-5.4 mg, i.p., on day -1, 1, 3, 7 and 10) significantly prolongs the survival time of porcine anterior islet xenografts by depleting CD4+ T cells in male CBA/H (H-2k) mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c (H-2d) mice (6-8 weeks)[1]
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Dosage:0.2 mg
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Administration:Intraperitoneal injection (i.p.), single dose on day 0
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Result:Significantly reduced the number of CD4+ T cells.
Significantly inceased the percentages of CD4+CD25+ T cells in peripheral Treg.
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Animal Model:1×106 EG7 cells injected C57BL/6 mice (6-8 weeks)[3]
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Dosage:5 mg/kg, combined with the OVA peptide vaccine (s.c., on day -14, -7, 0, and 6)
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Administration:Intraperitoneal injection (i.p.), on day -8 and 6
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Result:Significantly reduced the weight of spleen and liver.
Increased the infiltration of CD8+ T cells.
Reduced CD4+ T cells in non tumor liver tissue.
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Animal Model:Male CBA/H (H-2k) mice (7-9 weeks)[4]
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Dosage:0.2, 1.0, 4.2, 4.8 and 5.4 mg
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Administration:Intraperitoneal injection (i.p.), on day -1, 1, 3, 7 and 10
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Result:Efficiently depleted CD4+ T cells 2 weeks after transplantation.
Maintained low CD4+ T cell levels (9 %) at a high dose (5.4 mg) for 6 weeks after transplantation and the transplant remained alive.
NP_038516.1
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay, Research in vivo
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CD4 Antibody (GK1.5)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yi H, et al. Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74. [Content Brief]
[2]. Ni Q, et al. Gut microbiota dysbiosis orchestrates vitiligo-related oxidative stress through the metabolite hippuric acid. Microbiome. 2025 May 6;13(1):112. [Content Brief]
[3]. Fujinami N, et al. Enhancement of antitumor effect by peptide vaccine therapy in combination with anti-CD4 antibody: Study in a murine model. Biochem Biophys Rep. 2016 Feb 19;5:482-491. [Content Brief]
[4]. Simeonovic CJ, et al. Effect of GK1.5 monoclonal antibody dosage on survival of pig proislet xenografts in CD4+ T cell-depleted mice. Transplantation. 1990 May;49(5):849-56. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CD4 Antibody (GK1.5) Related Classifications
HY-P990792 Related Classifications
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