Anti-Mouse PD-L2/B7-DC Antibody (TY25)
Based on 1 Customer Validation
Anti-Mouse PD-L2/B7-DC Antibody (TY25) is a rat-derived IgG2a κ type antibody inhibitor, targeting to mouse PD-L2/B7-DC. Anti-Mouse PD-L2/B7-DC Antibody (TY25) blocks PD-L2 binding to PD-1 and blocks PD-1 signaling. Anti-Mouse PD-L2/B7-DC Antibody (TY25) can be used for the researches of inflammation and immunology, such as conjunctivitis and transplantation.
For research use only. We do not sell to patients.
- Purity : 96.71%
- Molecular Weight:150 kDa
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
PD-L2/B7-DC
In Vitro
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse PD-L2/B7-DC Antibody (TY25) (250 μg, i.p., 6 h before and 48 h following each allergen exposure) limits the severity of airway hyperresponsiveness in allergen-induced asthma mice models[2].
Anti-Mouse PD-L2/B7-DC Antibody (TY25) (500 μg, i.p., at days 0, 2, 4, 6, and 8) significantly increases eosinophil infiltration into the conjunctivain in ragweed pollen-induced experimental allergic conjunctivitis mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Orthotopic liver transplantation mice models[1]
-
Dosage:500 μg/mouse at day 0 and 250 μg/ mouse twice a week during 4 weeks
-
Administration:Intraperitoneally injection
-
Result:Inhibited the induction of spontaneous tolerance.
-
Animal Model:Mice were injected ragweed pollen into the left hind footpad and at the base of the tail[3]
-
Dosage:200 μg
-
Administration:Intraperitoneally injection, at days 0, 2, 4, 6, and 8
-
Result:Significantly increased IL-4, IL-5, IL-10, and IL-13 production by splenocytes.
Upregulated specific IgG1 and IgG2a levels.
Increased eosinophil infiltration into the conjunctiva.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
-
Product Image
Application
in vivo PD-L2 blockade; in vitro PD-L2 blockade; Immunohistochemistry (frozen); Flow cytometry
Chemical Information
-
Appearance Liquid
-
Molecular Weight 150 kDa
-
Color Colorless to light yellow
-
SMILES
[Anti-Mouse PD-L2/B7-DC Antibody (TY25)]
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (262 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
[1]. Morita M, et al. PD-1/B7-H1 interaction contribute to the spontaneous acceptance of mouse liver allograft. Am J Transplant. 2010 Jan;10(1):40-6. [Content Brief]
[2]. Lewkowich IP, et al. PD-L2 modulates asthma severity by directly decreasing dendritic cell IL-12 production. Mucosal Immunol. 2013 Jul;6(4):728-39. [Content Brief]
[3]. Fukushima A, et al. Involvement of programmed death-ligand 2 (PD-L2) in the development of experimental allergic conjunctivitis in mice. Br J Ophthalmol. 2006 Aug;90(8):1040-5. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse PD-L2/B7-DC Antibody (TY25) Related Classifications
HY-P990170 Related Classifications
Powered by Bioz