Antibacterial agent 307 hydrochloride 

Antibacterial agent 307 hydrochloride is an antibacterial agent. Antibacterial agent 307 hydrochloride shows MICs of 1-4 μg/mL against Gram-positive bacteria, moderate activity against Gram-negative bacteria, low hemolytic toxicity, and excellent plasma stability. Antibacterial agent 307 hydrochloride compromises bacterial membrane integrity via increased permeability and depolarization, induces endogenous ROS accumulation, causes cytoplasmic protein and nucleic acid leakage, and drives rapid bacterial cell death. Antibacterial agent 307 hydrochloride can be used for the research of bacterial infection^[1].

Antibacterial agent 307 (Compound 13e) (24 h) hydrochloride potently inhibits growth of gram-positive bacterial strains (S. aureus ATCC25923, S. aureus ATCC43300, E. faecalis ATCC29212, B. subtilis ATCC9372) with MIC values of 1, 2, 2, and 4 μg/mL, respectively, and exhibits weaker activity against gram-negative strains with MIC values of 16 and 32 μg/mL for E. coli ATCC25922 and P. aeruginosa ATCC27853, respectively^[1].
Antibacterial agent 307 (1-512 μg/mL; 1 h) hydrochloride exhibits low hemolytic toxicity toward sheep red blood cells, with an HC₅₀ of 95.01 μg/mL, resulting in less than 10% hemolysis at concentrations up to 64 μg/mL^[1].
Antibacterial agent 307 (1-128 μg/mL; 24 h) hydrochloride has low cytotoxicity toward LO2 human liver cells, with a CC₅₀ of 26.38 μg/mL, and a promising selectivity index of 89.21 against S. aureus ATCC25923^[1].
Antibacterial agent 307 (18 h) hydrochloride shows excellent plasma stability, maintaining an MBC of 8 μg/mL against S. aureus ATCC25923 after up to 6 h pre-incubation in 50% plasma, and retains potent bactericidal activity in mammalian fluids with MBC values of 8, 8, and 16 μg/mL in 50% plasma, 50% serum, and 50% blood, respectively^[1].
Antibacterial agent 307 (1-8 μg/mL; 24 h) hydrochloride exhibits rapid, dose-dependent bactericidal activity against S. aureus ATCC25923, reducing bacterial load by 4.51 log₁₀ CFU/mL within 8 h at 4 μg/mL and completely eradicating bacteria within 4 h at 8 μg/mL^[1].
Antibacterial agent 307 (0.5 μg/mL; 18 h per passage, 20 consecutive days) hydrochloride has a low propensity to induce resistance in S. aureus ATCC25923, as its MIC remains nearly unchanged within 17 days of serial passaging^[1].
Antibacterial agent 307 (0.5-16 μg/mL; 24 h) hydrochloride inhibits S. aureus ATCC25923 biofilm formation by up to 73.6% at 16 μg/mL and disrupts pre-formed biofilms by up to 38.0% at 16 μg/mL, demonstrating concentration-dependent anti-biofilm activity^[1].
Antibacterial agent 307 (1-8 μg/mL; 30 min) hydrochloride disrupts the membrane polarization of S. aureus ATCC25923 in a concentration-dependent manner, causing a 6.8-fold increase in DiSC₃(5) fluorescence intensity at 8 μg/mL^[1].
Antibacterial agent 307 (1-8 μg/mL; 30 min) hydrochloride increases the membrane permeability of S. aureus ATCC25923 in a concentration-dependent manner, causing a 2.6-fold increase in PI fluorescence intensity at 8 μg/mL within 24 min^[1].
Antibacterial agent 307 (1-16 μg/mL; 30 min) hydrochloride induces concentration-dependent intracellular oxidative stress in S. aureus ATCC25923, increasing ROS levels by 2-fold at 16 μg/mL relative to the control^[1].
Antibacterial agent 307 (1-16 μg/mL; 4 h) hydrochloride disrupts the membrane of S. aureus ATCC25923, causing concentration-dependent leakage of intracellular DNA and protein, with a 4.6-fold increase in extracellular DNA and 2.2-fold increase in protein leakage at 16 μg/mL relative to the control^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Antibacterial agent 307 (Compound 13e) (2.5-5.0 mg/kg; s.c.; single dose) hydrochloride exhibits potent antibacterial efficacy in a mouse skin abscess model infected by S. aureus ATCC25923., achieving a 99.88% reduction in bacterial load at the 5.0 mg/kg dose and greatly relieving tissue inflammation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

560.84

C₂₃H₂₆BrClF₃N₅O

BrC1=CC=C2C(N(CC3=CC=C(C(F)(F)F)C=C3)C=C2C(NCCCCCNC(N)=N)=O)=C1.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Qin Y, et al. Development of marine-derived indole derivatives as novel antibacterial agents against gram-positive bacteria. Bioorg Chem. 2025 Nov 26;168:109300.  [Content Brief]