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Cat. No.: HY-P83848A

AMPK beta 2 Antibody (YA3545)(PBS only)

Name: AMPK beta 2 Antibody (YA3545)(PBS only)
Brand: MedChemExpress
SKU: HY-P83848A
Availability: PreOrder

AMPK beta 2 Antibody (YA3545) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AMPK beta 2.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	FC FC: Flow Cytometry	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:200-1:1000	1:200-1:1000	1:200-1:400	1:10000

Description

AMPK beta 2 Antibody (YA3545) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AMPK beta 2.

Host Mouse
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 30 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 30.3 kDa

Immunogen

Purified recombinant fragment of human PRKAB2 (AA: 1-120) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG1

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

AMPK beta 2 is a Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3)
Isoforms & Post-Translational Modification

O43741 has 2 isomers: O43741-1: 30302 Da (predicted); O43741-2: 21485 Da (predicted).
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK
Subunit

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3)
SwissProt ID

O43741
Gene ID

5565 [NCBI]