APAF1 Antibody (YA3503)(PBS only)
(Synonyms: CED4; APAF-1)APAF1 Antibody (YA3503) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to APAF1.
-
Host:
Mouse
-
Isotype:
IgG2b
-
Application:
ICC/IF, FC, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS, pH 7.4.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:100-1:500 | 1:200-1:400 | 1:10000 |
Product Details
APAF1 Antibody (YA3503) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to APAF1.
-
Host Mouse
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 142 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 141.8 kDa
Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG2b
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS, pH 7.4.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
APAF1 is an Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis
-
Subcellular Localization
Cytoplasm
-
Expression
Tissue_specificity: It is widely distributed. Its expression levels are highest in human spleen and peripheral blood leukocytes, and it is also expressed in the fetal brain, kidneys, and lungs. Isomer 1 is expressed in the heart, kidneys, and liver.
Induction: By E2F and p53/TP53 in apoptotic neurons (PubMed:11389439). Translation is inhibited by HNRPA1, which binds to the IRES of APAF1 mRNAs (PubMed:31498791). -
Subunit
Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227)
-
SwissProt ID
-
Synonyms
CED4; APAF-1
Documentation
Powered by Bioz
