APAF1 Antibody (YA3503)(PBS only)

Cat. No.: HY-P83806A: Data Sheet User Guide for Antibodies
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APAF1 Antibody (YA3503) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to APAF1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

APAF1 Antibody (YA3503) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to APAF1.

Host

Mouse

Molecular Weight

Predicted band size: 141.8 kDa;

Observed band size: 142 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O14727

Gene ID

317 [NCBI]

Immunogen

Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity: It is widely distributed. Its expression levels are highest in human spleen and peripheral blood leukocytes, and it is also expressed in the fetal brain, kidneys, and lungs. Isomer 1 is expressed in the heart, kidneys, and liver.

Induction: By E2F and p53/TP53 in apoptotic neurons (PubMed:11389439). Translation is inhibited by HNRPA1, which binds to the IRES of APAF1 mRNAs (PubMed:31498791).

Subunit：Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A (PubMed:25716227)

Synonyms

CED4; APAF-1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/cho-cell.html [Content Brief]