Apc11/ANAPC11 Antibody (YA7108)

Cat. No.: HY-P87420: Data Sheet User Guide for Antibodies
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Apc11/ANAPC11 Antibody (YA7108) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Apc11/ANAPC11.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Apc11/ANAPC11 Antibody (YA7108) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Apc11/ANAPC11.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 9.8kDa;

Species Reactivity

Human

SwissProt ID

Q9NYG5

Immunogen

Purified recombinant fragment of human ANAPC11 (AA: 1-196) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:11739784, PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). May recruit the E2 ubiquitin-conjugating enzymes to the complex (PubMed:11739784, PubMed:18485873)

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte

Isoforms & Post-Translational Modification：Q9NYG5 has two isomers: Q9NYG5-1: 9841 Da (predicted); Q9NYG5-2: 20644 Da (predicted).
Auto-ubiquitinated

Subunit：The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1

Database

SwissProt: SwissProt: Q9NYG5

Documentation

User Guide for Antibodies (1077 KB)