ATP6V1F Antibody (YA8304)

Cat. No.: HY-P88620: Ficha de datos User Guide for Antibodies
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ATP6V1F Antibody (YA8304) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP6V1F.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

ATP6V1F Antibody (YA8304) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to ATP6V1F.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 13.2 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q16864

Immunogen

Full length human recombinant protein of human ATP6V1F produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity)

Subcellular Localization：Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Cytoplasmic vesicle, clathrin-coated vesicle membrane

Isoforms & Post-Translational Modification：Q16864 has two isomers: Q16864-1: 13370 Da (predicted); Q16864-2: 16402 Da (predicted).

Subunit：V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:33065002)

Synonyms

ATP6S14; VATF; Vma7

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/hepg2-cell.html [Content Brief]