BACH1 Antibody (YA4463)(PBS only)

Cat. No.: HY-P84766A: Data Sheet User Guide for Antibodies
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BACH1 Antibody (YA4463) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BACH1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BACH1 Antibody (YA4463) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BACH1.

Host

Mouse

Molecular Weight

Predicted band size: 141 kDa;

Observed band size: 105 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BX63

Gene ID

83990 [NCBI]

Immunogen

Purified recombinant fragment of human BRIP1 (AA: 904-986) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability (PubMed:11301010, PubMed:14983014, PubMed:16116421, PubMed:16153896, PubMed:17596542, PubMed:36608669). Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination (PubMed:14983014, PubMed:16153896). Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 (PubMed:14983014, PubMed:16153896). Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease (PubMed:16116421, PubMed:36608669). Can unwind RNA:DNA substrates (PubMed:14983014). Unwinds G-quadruplex DNA; unwinding requires a 5'-single stranded tail (PubMed:18426915, PubMed:20639400)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is widely expressed, with the highest expression level in the testes.

Isoforms & Post-Translational Modification：Q9BX63 has 2 isomers: Q9BX63-1: 140867 Da (predicted); Q9BX63-2: 112466 Da (predicted).
Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated;Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling

Subunit：Interacts with the replication protein A complex (RPA) via the RPA1 subunit; following DNA damage they colocalize in foci in the nucleus (PubMed:17596542). Binds directly to the BRCT domains of BRCA1 (PubMed:15125843). Interacts with the CIA complex components CIAO1, CIAO2B and MMS19 (PubMed:23585563)

Synonyms

OF; BACH1; FANCJ

Documentation

User Guide for Antibodies (1077 KB)