BOB1 Antibody (YA5672)

Cat. No.: HY-P85980: Data Sheet User Guide for Antibodies
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BOB1 Antibody (YA5672) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BOB1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

BOB1 Antibody (YA5672) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to BOB1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse,

SwissProt ID

Q16633

Gene ID

5450 [NCBI]

Immunogen

Synthesized peptide derived from human BOB.1 AA range: 150-256

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:B cell specific (PubMed:7779176, PubMed:7859290) . It is mainly detected in the spleen, but is also found in the thymus, peripheral blood leukocytes, and small intestine (PubMed:7779176, PubMed:7859290) .

Subunit：Interacts with POU2F1/OCT1 and POU2F2/OCT2; the interaction increases POU2F1 and POU2F2 transactivation activity

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/st-4-cell.html [Content Brief]