Thrombopoietin Receptor Antibody (YA3994)
(Synonyms: MPL; MPLV; TPOR; C-MPL; THCYT2)
Based on 1 Customer Validation
Thrombopoietin Receptor Antibody (YA3994) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Thrombopoietin Receptor.
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Host:
Mouse
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Isotype:
IgG
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Application:
FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:200-1:400 | 1:10000 |
Product Details
Thrombopoietin Receptor Antibody (YA3994) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Thrombopoietin Receptor.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 85 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 71 kDa
Purified recombinant fragment of human CD110 (AA: extra 26-175) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Thrombopoietin (TPO) is the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
TPO binds to the thrombopoietin receptor (TPOr, also termed c-mpl) on platelets, megakaryocytes, and pluripotent stem cells leading to inhibition of apoptosis of stem cells and megakaryocytes; increased megakaryocyte number, size, and ploidy; increased rate of megakaryocyte maturation and platelet count; and decreased platelet threshold for activation by ADP and collagen.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Golgi apparatus; Cell surface
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Expression
Tissue_specificity:It is expressed at low levels in a large number of hematopoietic cells. Isomer 1 and isomer 2 are always co-expressed. -
Isoforms & Post-Translational Modification
P40238 has 2 isomers: P40238-1: 71245 Da (predicted); P40238-2: 65521 Da (predicted).
Phosphorylated at Tyr-591 in response to THPO stimulation;Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination -
Subunit
Homodimer (PubMed:32029621, PubMed:25538044). Interacts with ATXN2L (PubMed:11784712). Interacts with JAK2 and TYK2; these interactions increase MPL localization to the cell membrane (PubMed:15899890). Interacts with THPO (PubMed:37633268). Interacts with SHIP/INPP5D (PubMed:24607955). Interacts with BTK (PubMed:24607955). Interacts with SYK; this interaction negatively regulates THPO-mediated ERK1/2 signaling (PubMed:24607955)
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SwissProt ID
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Synonyms
MPL; MPLV; TPOR; C-MPL; THCYT2
Documentation
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