CD11b Antibody (YA3751)(PBS only)

Cat. No.: HY-P84054A: Data Sheet User Guide for Antibodies
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CD11b Antibody (YA3751) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD11b.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD11b Antibody (YA3751) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD11b.

Host

Mouse

Molecular Weight

Predicted band size: 127 kDa;

Observed band size: 127 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P11215

Gene ID

3684 [NCBI]

Immunogen

Purified recombinant fragment of human ITGAM (AA: 623-728) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is mainly expressed in monocytes and granulocytes (PubMed: 1346576) . It is also expressed in neutrophils (protein level) (PubMed: 21193407) .

Isoforms & Post-Translational Modification：P11215 has 2 isomers: P11215-1: 127179 Da (predicted); P11215-2: 127307 Da (predicted).

Subunit：Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18 (PubMed:21193407). Interacts with JAM3 (PubMed:12208882, PubMed:15194813). Interacts with THBD (PubMed:27055590). Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance (PubMed:20008295, PubMed:9558116). Interacts with TMEM268; this interaction inhibits ITGAM degradation via the endosome-lysosome pathway (PubMed:38730209)

Synonyms

CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Documentation

User Guide for Antibodies (1077 KB)