CD15 Antibody (YA4774)

Cat. No.: HY-P85082: Data Sheet User Guide for Antibodies
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CD15 Antibody (YA4774) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD15.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD15 Antibody (YA4774) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD15.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

P22083

Gene ID

2526 [NCBI]

Immunogen

Synthesized peptide of human CD15.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:1702034, PubMed:1716630, PubMed:29593094). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:1716630, PubMed:29593094). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:11278338, PubMed:1716630); Does not generate Lewis X antigens

Subcellular Localization：Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein

Expression：
Tissue_specificity:It is expressed at low levels in bone marrow mesenchymal stem cells; it is also expressed in immature promyelocytes in umbilical cord blood and in peripheral blood myeloid and lymphoid cell populations.

Isoforms & Post-Translational Modification：P22083 has 2 isomers: P22083-1: 59084 Da (predicted); P22083-2: 45629 Da (predicted).

Synonyms

FUT4; ELFT; FCT3A; Alpha-; 1; 3; -fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase

Documentation

User Guide for Antibodies (1077 KB)