CD162 Antibody (YA4662)

Cat. No.: HY-P84970: Data Sheet User Guide for Antibodies
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CD162 Antibody (YA4662) is a non-conjugated antibody, targeting to CD162.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD162 Antibody (YA4662) is a non-conjugated antibody, targeting to CD162.

Host

Mouse

Clonality

Monoclonal Antibody

Species Reactivity

Human

SwissProt ID

Q14242

Gene ID

6404 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture; (Microbial infection) Acts as a receptor for enterovirus 71

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is expressed on neutrophils, monocytes, and most lymphocytes.

Isoforms & Post-Translational Modification：Q14242 has 2 isomers: Q14242-1: 43201 Da (predicted); Q14242-2: 44648 Da (predicted).
Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation;Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling

Subunit：Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparently not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20. Interacts with MSN and SYK; mediates the activation of SYK by SELPLG. Interacts with HAVCR1 (PubMed:24703780)

Synonyms

CLA; CD162; PSGL1; PSGL-1; PSGL-1; p-selectin glycoprotein ligand-1; CD 162 antibody; CD162 antibody; CD162 antigen antibody; CLA antibody; Cutaneous lymphocyte associated associated antigen antibody; P selectin glycoprotein ligand 1 antibody; P selectin glycoprotein ligand 1 precursor antibody; P-selectin glycoprotein ligand 1 antibody; PSGL 1 antibody; PSGL-1 antibody; PSGL1 antibody; Selectin P ligand antibody; SELPL_HUMAN antibody; SELPLG antibody;

Documentation

User Guide for Antibodies (1077 KB)