PD-L1 Antibody (YA4539)
(Synonyms: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1,CD274)PD-L1 Antibody (YA4539) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L1.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
PD-L1 Antibody (YA4539) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 46 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD274 (PD-L1) is an important immune checkpoint ligand protein belonging to the B7 family of immunoregulatory molecules. By binding to PD-1 on the surface of T cells, PD-L1 inhibits T-cell activation and immune responses; it is a key mechanism of tumor immune escape and a major target for current immune checkpoint therapies.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Single-pass type I membrane protein; Recycling endosome membrane; Single-pass type I membrane protein; Nucleus; Cell membrane; Single-pass type I membrane protein; Endomembrane system; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:It is highly expressed in the heart, skeletal muscle, placenta, and lungs. It is weakly expressed in the thymus, spleen, kidneys, and liver. It is expressed on activated T cells and B cells, dendritic cells, keratinocytes, and monocytes; it is widely expressed, with the highest expression in the lungs, liver, pituitary gland, and various peripheral blood cells (including neutrophils and certain subtypes of lymphocytes and myeloid cells) .
Induction:Up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. Up-regulated in B-cells activated by surface Ig cross-linking -
Isoforms & Post-Translational Modification
CD274 (PD-L1) has 4 isomers: Q9NZQ7-1: 33275 Da (predicted); Q9NZQ7-2: 20246 Da (predicted); Q9NZQ7-3: 20454 Da (predicted); Q9NZQ7-4: 28050 Da (predicted).
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8; leading to degradation. Deubiquitinated by USP22; leading to stabilization -
Subunit
Interacts with PDCD1. Interacts (via transmembrane domain) with CMTM4 and CMTM6. Interacts with (phosphorylated) STAT3; promoting nuclear translocation. Interacts with CD80
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SwissProt ID
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Synonyms
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1,CD274
Documentation
References
[1]. Freeman GJ, et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34. [Content Brief]
[2]. Mezzadra R, et al. Identification of CMTM6 and CMTM4 as PD-L1 protein regulators. Nature. 2017 Sep 7;549(7670):106-110. [Content Brief]
[3]. Burr ML, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017 Sep 7;549(7670):101-105. [Content Brief]
[4]. Huang X, et al. USP22 Deubiquitinates CD274 to Suppress Anticancer Immunity. Cancer Immunol Res. 2019 Oct;7(10):1580-1590. [Content Brief]
[5]. Kennedy A, et al. The CTLA-4 immune checkpoint protein regulates PD-L1:PD-1 interaction via transendocytosis of its ligand CD80. EMBO J. 2023 Mar 1;42(5):e111556. [Content Brief]
[6]. Dong H, et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999 Dec;5(12):1365-9. [Content Brief]
[7]. Hou J, et al. PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis. Nat Cell Biol. 2020 Oct;22(10):1264-1275. [Content Brief]
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