ICOS Antibody (YA4676)

Cat. No.: HY-P84984: Data Sheet User Guide for Antibodies
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ICOS Antibody (YA4676) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ICOS.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ICOS Antibody (YA4676) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ICOS.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

Q9Y6W8

Gene ID

29851 [NCBI]

Immunogen

Purified recombinant Human CD278

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:33033255). Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Secreted

Expression：
Tissue_specificity:Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

Induction:By phorbol myristate acetate (PMA) and ionomycin. Up-regulated early on T-cells and continues to be expressed into the later phases of T-cell activation.

Isoforms & Post-Translational Modification：Q9Y6W8 has 2 isomers: Q9Y6W8-1: 22625 Da (predicted); Q9Y6W8-2: 19127 Da (predicted).
N-glycosylated

Subunit：Homodimer; disulfide-linked. Interacts with ICOSLG (PubMed:33033255). Interacts with PIK3R1 (PubMed:30523347). Interacts with TBK1; this interaction is critical for the maturation of T follicular regulatory cells (PubMed:27135603)

Synonyms

ICOS; AILIM; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; CD278 nanobody; ICOS nanobody;

Documentation

User Guide for Antibodies (1077 KB)