CD80 Antibody (YA3963)(PBS only)
(Synonyms: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1)CD80 Antibody (YA3963) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD80.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human, Mouse, Rat, Rabbit
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:100-1:500 | 1:200-1:400 | 1:10000 |
Product Details
CD80 Antibody (YA3963) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD80.
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Host Mouse
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Species ReactivityHuman, Mouse, Rat, Rabbit
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Observed Molecular WeightObserved band size: 50-75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Purified recombinant fragment of human CD80 (AA: 35-288) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD28/CD80 is a co-stimulatory molecule belonging to the immunoglobulin superfamily that plays a crucial role in T-lymphocyte activation. CD80 interacts with the CD28 receptor-constitutively expressed on the surface of T cells-to serve as a primary co-stimulatory signal that enhances MHC/TCR signaling in naive T cells. This triggers the activation of various signaling pathways, such as NF-κB or MAPK, ultimately leading to the production of multiple cytokines. Furthermore, the CD28/CD80 co-stimulatory signal promotes T-cell glucose metabolism and ATP synthesis by activating the PI3K/Akt signaling pathway. CD80 also acts as a regulator of the PD-L1/PDCD1 interaction, limiting the excessive activation of PD-L1 and its inhibitory effects during immune responses. Expressed on the surface of B cells, CD80 plays a pivotal regulatory role in the interactions between B cells and T cells during both early and late germinal center reactions.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:It is expressed on activated B cells, macrophages, and dendritic cells. -
Isoforms & Post-Translational Modification
CD80 has 3 isomers: P33681-1: 33048 Da (predicted); P33681-2: 29413 Da (predicted); P33681-3: 18761 Da (predicted).
Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization -
Subunit
Homodimer. Interacts with CTLA4; this interaction inhibits T-cell activation. Interacts with PDL1/CD274; this interaction blocks PDL1/PDCD1 binding and thus PDL1/CD274 inhibitory function. Interacts with CD28
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SwissProt ID
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Synonyms
B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Documentation
References
[1]. Lu B, et al. zDHHC20-driven S-palmitoylation of CD80 is required for its costimulatory function. Acta Pharmacol Sin. 2024 Jun;45(6):1214-1223. [Content Brief]
[2]. Collins AV, et al. The interaction properties of costimulatory molecules revisited. Immunity. 2002 Aug;17(2):201-10. [Content Brief]
[3]. Boulougouris G, et al. IL-2-independent activation and proliferation in human T cells induced by CD28. J Immunol. 1999 Aug 15;163(4):1809-16. [Content Brief]
[4]. Frauwirth KA, et al. The CD28 signaling pathway regulates glucose metabolism. Immunity. 2002 Jun;16(6):769-77. [Content Brief]
[5]. Kennedy A, et al. The CTLA-4 immune checkpoint protein regulates PD-L1:PD-1 interaction via transendocytosis of its ligand CD80. EMBO J. 2023 Mar 1;42(5):e111556. [Content Brief]
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