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CHRNA6 Antibody (YA4301)

(Synonyms: CHNRA6)
Customer Review

Based on 1 Customer Validation

CHRNA6 Antibody (YA4301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA6.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • CHRNA6 Antibody (YA4301) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

CHRNA6 Antibody (YA4301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA6.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718947

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    CHRNA6 component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (Probable). CHRNA6 forms pentameric channels with CHRNB2, CHRNB3 and CHRNA4 that exhibit high sensitivity to ACh and nicotine and are predominantly expressed in only a few brain areas, including dopaminergic neurons, norepirephrine neurons and cells of the visual system. nAChrs containing CHRNA6 subunits mediate endogenous cholinergic modulation of dopamine and gamma-aminobutyric acid (GABA) release in response to nicotine at nerve terminals[1].

  • Subcellular Localization

    Synaptic cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    Q15825 has 2 isomers: Q15825-1: 56898 Da (predicted); Q15825-2: 55018 Da (predicted).

  • Subunit

    Neuronal AChR is composed of two different types of subunits: alpha and non-alpha (beta). CHRNA6/alpha-6 subunit can be combined to CHRNB2/beta-2, CHRNA4/alpha-4 and CHRNB3/beta-3 to give rise to functional receptors (PubMed:16835356). Heteropentamers containing CHRNB3 have an stoichiometry of (CHRNA6:CHRNB2)2:CHRNB3 (PubMed:16835356). Interacts with LYPD6 (PubMed:27344019)

  • SwissProt ID

    Q15825

  • Gene ID
    8973 [NCBI]
  • Synonyms

    CHNRA6

CHRNA6 Antibody (YA4301) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    1/16
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • CHRNA6 Antibody (YA4301) Product Image
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • Experimental Validation Results for CHRNA6 Antibody (YA4301)
  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Brain tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using CHRNA6 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84604, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P
  • CHRNA6 Antibody (YA4301) Product Image IHC-P