CLTA Antibody (YA8053)

(Synonyms: LCA)

CLTA Antibody (YA8053) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLTA.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-4000 1:2000-2500

Product Details

Description

CLTA Antibody (YA8053) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLTA.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 23.5 kDa
Immunogen

Full length human recombinant protein of human CLTA produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human urothelial cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CLTA Antibody (YA8053)
    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using CLTA Antibody (HY-P88369, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CLTA is a Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge[1][2].

  • Subcellular Localization

    Cytoplasmic vesicle membrane,Membrane, coated pit,Cytoplasm, cytoskeleton, spindle

  • Isoforms & Post-Translational Modification

    P09496 has 5 isomers: P09496-1: 27077 Da (predicted); P09496-2: 23662 Da (predicted); P09496-3: 25676 Da (predicted); P09496-4: 24993 Da (predicted); P09496-5: 17687 Da (predicted).

  • Subunit

    Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains

  • SwissProt ID

    P09496

  • Synonyms

    LCA

CLTA Antibody (YA8053) Related Classifications

MOQ
Minimum order quantity
100 mg

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