DNAJB2 Antibody (YA8517)

Cat. No.: HY-P88833: Data Sheet User Guide for Antibodies
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DNAJB2 Antibody (YA8517) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DNAJB2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DNAJB2 Antibody (YA8517) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DNAJB2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 30.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P25686

Immunogen

Full length human recombinant protein of human DNAJB2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:22219199, PubMed:7957263). In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278)

Subcellular Localization：Cytoplasm,Nucleus,Endoplasmic reticulum membrane

Expression：
Tissue_Specificity: More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:12754272, PubMed:1599432). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272)

Isoforms & Post-Translational Modification：P25686 has two isomers: P25686-3: 35580 Da (predicted); P25686-2: 30569 Da (predicted).
Ubiquitinated by STUB1; does not lead to proteasomal degradation

Subunit：Interacts with HSP70 (HSPA1A or HSPA1B) (PubMed:21625540, PubMed:22219199)

Synonyms

CMT2T; DSMA5; HSJ-1; HSJ1; HSPF3

Documentation

User Guide for Antibodies (1077 KB)