Dnmt3b Antibody (YA4550)(PBS only)

Cat. No.: HY-P84853A: Data Sheet User Guide for Antibodies
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Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Dnmt3b Antibody (YA4550) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Dnmt3b.

Host

Mouse

분자량

Predicted band size: 96 kDa;

Observed band size: 96 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UBC3

Gene ID

1789 [NCBI]

Immunogen

Purified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Universally present; highly expressed in fetal liver, heart, kidney, and placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isomer 1 is expressed in all tissues except brain, skeletal muscle, and peripheral blood mononuclear cells; isomer 3 is universally present; isomer 4 is expressed in all tissues except brain, skeletal muscle, lung, and prostate; isomer 5 is detected only in the testes, with extremely low expression levels in the brain and prostate.

Isoforms & Post-Translational Modification：Q9UBC3 has 8 isomers: Q9UBC3-1: 95751 Da (predicted); Q9UBC3-2: 93407 Da (predicted); Q9UBC3-3: 86177 Da (predicted); Q9UBC3-4: 80845 Da (predicted); Q9UBC3-5: 88572 Da (predicted); Q9UBC3-6: 94689 Da (predicted); Q9UBC3-7: 77719 Da (predicted); Q9UBC3-8: 81311 Da (predicted).
Sumoylated;Citrullinated by PADI4

Subunit：Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex

Synonyms

ICF; ICF1; M.HsaIIIB

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h226-cell.html