EIF1 Antibody (YA8460)
(Synonyms: A121; EIF-1; EIF1A; ISO1; SUI1)EIF1 Antibody (YA8460) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to EIF1.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ICC/IF, FC
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Reactivity :
Human, Rat
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:150-500 | 1:100-250 | 1:100 |
Product Details
EIF1 Antibody (YA8460) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to EIF1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Calculated Molecular Weight Predicted band size: 12.6 kDa
Full length human recombinant protein of human EIF1 produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
eIF1 is a small eukaryotic translation initiation factor that binds the 40S ribosomal subunit and supports accurate start-codon selection during protein synthesis[1]. Mechanistically, eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes after start-codon recognition and eIF1 release[2]. eIF1 dissociation from the preinitiation complex permits phosphate release from eIF2 and triggers downstream initiation events[3]. Mutations that weaken eIF1 binding reduce start-codon fidelity by increasing eIF1 release at non-AUG codons[3]. Structural studies place eIF1 on the 40S subunit and define its interactions with ribosomal RNA and initiation-factor partners[4][5]. Compared with eIF1A, eIF1 primarily gates start-codon recognition and phosphate release, whereas eIF1A modulates eIF1 dissociation and stabilizes the codon-anticodon duplex during recognition[2][6]. In disease models, the related isoform EIF1AX/eIF1A carries recurrent somatic mutations in uveal melanoma and cooperates with RAS mutations in advanced thyroid cancer models, but these findings should not be transferred directly to eIF1 without target-specific evidence[7][8]. No well-supported selective eIF1 agonists or inhibitors were identified in the cited literature.
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Subcellular Localization
Cytoplasm
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Isoforms & Post-Translational Modification
P41567: 113 amino acids, molecular weight 12732 Da.
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Subunit
Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex)
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SwissProt ID
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Synonyms
A121; EIF-1; EIF1A; ISO1; SUI1
Documentation
References
[1]. Fletcher CM, et al. Structure and interactions of the translation initiation factor eIF1. EMBO J. 1999 May 4;18(9):2631-7. [Content Brief]
[2]. Passmore LA, et al. The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome. Mol Cell. 2007 Apr 13;26(1):41-50. [Content Brief]
[3]. Nanda JS, et al. eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation. J Mol Biol. 2009 Nov 27;394(2):268-85. [Content Brief]
[4]. Rabl J, et al. Crystal structure of the eukaryotic 40S ribosomal subunit in complex with initiation factor 1. Science. 2011 Feb 11;331(6018):730-6. [Content Brief]
[5]. Weisser M, et al. The crystal structure of the eukaryotic 40S ribosomal subunit in complex with eIF1 and eIF1A. Nat Struct Mol Biol. 2013 Aug;20(8):1015-7. [Content Brief]
[6]. Hussain T, et al. Structural changes enable start codon recognition by the eukaryotic translation initiation complex. Cell. 2014 Oct 23;159(3):597-607. [Content Brief]
[7]. Martin M, et al. Exome sequencing identifies recurrent somatic mutations in EIF1AX and SF3B1 in uveal melanoma with disomy 3. Nat Genet. 2013 Aug;45(8):933-6. [Content Brief]
[8]. Krishnamoorthy GP, et al. EIF1AX and RAS Mutations Cooperate to Drive Thyroid Tumorigenesis through ATF4 and c-MYC. Cancer Discov. 2019 Feb;9(2):264-281. [Content Brief]
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