EXOSC3 Antibody (YA8581)

Cat. No.: HY-P88897: Fiche technique User Guide for Antibodies
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EXOSC3 Antibody (YA8581) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EXOSC3.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

EXOSC3 Antibody (YA8581) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EXOSC3.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 29.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NQT5

Immunogen

Full length human recombinant protein of human EXOSC3 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Pureté	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5

Subcellular Localization：Cytoplasm,Nucleus, nucleolus,Nucleus

Isoforms & Post-Translational Modification：Q9NQT5 has two isomers: Q9NQT5-1: 29572 Da (predicted); Q9NQT5-2: 17248 Da (predicted).

Subunit：Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC5 and EXOSC9 (PubMed:29906447, PubMed:30047866)

Synonyms

bA3J10.7; CGI-102; hRrp-40; p10; PCH1B; RRP40; Rrp40p

Documentation

User Guide for Antibodies (1077 KB)