FOXM1 Antibody (YA4205)
(Synonyms: MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2)FOXM1 Antibody (YA4205) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXM1.
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Host:
Mouse
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Isotype:
IgG
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Application:
FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:200-1:400 | 1:10000 |
Product Details
FOXM1 Antibody (YA4205) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FOXM1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 84 kDa
Purified recombinant fragment of human FOXM1 (AA: 649-748) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Forkhead box protein M1 (FOXM1, also known as Trident, WIN, HFH-11, and MPP2; 27-30) is a member of the FOX family of transcription factors. The expression and activity of FoxM1 is important for the correct coupling of DNA synthesis to mitosis. FOXM1 is ubiquitously expressed in all proliferating mammalian cells, with its expression levels rising at the start of DNA synthesis and persisting until the end of mitosis. In M-phase, FOXM1 is phosphorylated and degraded before M-phase exit. FOXM1 has been suggested to play a role in normal coupling of S-phase to M-phase during cell cycle progression. FOXM1 enhances tumor metastasis in lung carcinoma, breast adenocarcinoma, pancreatic carcinoma, prostate carcinoma, colorectal cancer, ovarian carcinoma, cervical and nasopharyngeal carcinoma, ESCC and glioma[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:This gene is expressed in the thymus, testes, small intestine, colon, and ovary. It appears to be expressed only in adult organs containing proliferating/cycled cells, or in response to growth factor stimulation. It is also expressed in tumor-derived epithelial cell lines. It is not expressed in quiescent cells. Isomer 2 is highly expressed in the testes.
Induction:Induced during liver regeneration and oxidative stress -
Isoforms & Post-Translational Modification
Q08050 has 3 isomers: Q08050-1: 84283 Da (predicted); Q08050-2: 82685 Da (predicted); Q08050-3: 88596 Da (predicted).
Phosphorylated in M (mitotic) phase (PubMed:17101782, PubMed:19160488, PubMed:30139873). Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair (PubMed:17101782). Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1 (PubMed:19160488, PubMed:30139873). Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators (PubMed:19160488). Phosphorylated by GSK3B leading to ubiquitination and proteasomal degradation (PubMed:26912724);Ubiquitinated in a FBXW7-dependent manner leading to proteasomal degradation -
Subunit
Interacts with PINT87aa which is encoded by the circular form of the long non-coding RNA LINC-PINT; the interaction inhibits FOXM1-mediated transcription of PHB2
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SwissProt ID
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Synonyms
MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
Documentation
[1]. Bicknell KA. Forkhead (FOX) transcription factors and the cell cycle: measurement of DNA binding by FoxO and FoxM transcription factors. Methods Mol Biol. 2005;296:247-62. [Content Brief]
[2]. Cheng L, et al. FOXM 1 induces Vasculogenic mimicry in esophageal cancer through β-catenin /Tcf4 signaling. Diagn Pathol. 2020 Feb 8;15(1):14. [Content Brief]
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