Frizzled 4/CD344 Antibody (YA7110)

Art. -Nr.: HY-P87422: Data Sheet User Guide for Antibodies
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Frizzled 4/CD344 Antibody (YA7110) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Frizzled 4/CD344.

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Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Frizzled 4/CD344 Antibody (YA7110) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Frizzled 4/CD344.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 60kDa;

Species Reactivity

Human

SwissProt ID

Q9ULV1

Immunogen

Purified recombinant fragment of human CD344 (AA: extra 37-222) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Reinheit	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Receptor for Wnt proteins (PubMed:30135577). Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed:30135577). Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues

Subcellular Localization：Cell membrane

Expression：
Tissue_Specificity: Almost ubiquitous (PubMed:10544037). Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney (PubMed:10544037). Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver (PubMed:10544037)

Isoforms & Post-Translational Modification：Q9ULV1: 537 amino acids, molecular weight 59881 Da.

Subunit：Interacts with MAGI3 and NDP (By similarity)

Database

SwissProt: SwissProt: Q9ULV1

Synonyms

CD344, Frizzled-4, Fz-4, hFz4, FzE4, FZD4

Dokumentation

User Guide for Antibodies (1077 KB)