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G9P/CD158D Antibody (YA7099)

(Synonyms: CD158d, CD158D, KIR103AS, KIR2DL4, Killer cell immunoglobulin-like receptor 2DL4, CD158 antigen-like family member D, G9P, Killer cell inhibitory receptor 103AS, MHC class I NK cell receptor KIR103AS, KIR-103AS)
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Based on 1 Customer Validation

G9P/CD158D Antibody (YA7099) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to G9P/CD158D.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for G9P/CD158D Antibody (YA7099)
  • G9P/CD158D Antibody (YA7099) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:400 1:10000

Product Details

Description

G9P/CD158D Antibody (YA7099) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to G9P/CD158D.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 41.5kDa;
Species Reactivity Database
Immunogen

Purified recombinant fragment of human CD158D (AA: extra 22-120) expressed in E. Coli.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for G9P/CD158D Antibody (YA7099)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD158D Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    G9P/CD158D receptor for non-classical major histocompatibility class Ib HLA-G molecules. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M). In decidual NK cells, binds peptide-bound HLA-G-B2M complex and triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells. Upon interaction with peptide-bound HLA-G-B2M, initiates signaling from the endosomal compartment leading to downstream activation of PRKDC-XRCC5 and AKT1, and ultimately triggering NF-kappa-B-dependent pro-inflammatory response[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane,Early endosome membrane

  • Expression


    Tissue_Specificity: Expressed in decidual NK cells and innate lymphoid cell type I (ILC1) (PubMed:29262349). Expressed in a subset of peripheral NK cells (PubMed:19304799)

  • Isoforms & Post-Translational Modification

    Q99706 has 6 isomers: Q99706-1: 41487 Da (predicted); Q99706-2: 39699 Da (predicted); Q99706-3: 37356 Da (predicted); Q99706-4: 35568 Da (predicted); Q99706-5: 33591 Da (predicted); Q99706-6: 24917 Da (predicted).

  • Subunit

    Interacts with peptide-bound HLA-G-B2M heterotrimeric complex (PubMed:10190900, PubMed:16366734)

  • SwissProt ID

    Q99706

  • Synonyms

    CD158d, CD158D, KIR103AS, KIR2DL4, Killer cell immunoglobulin-like receptor 2DL4, CD158 antigen-like family member D, G9P, Killer cell inhibitory receptor 103AS, MHC class I NK cell receptor KIR103AS, KIR-103AS

G9P/CD158D Antibody (YA7099) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD158D Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • G9P/CD158D Antibody (YA7099) Product Image
    1/16
  • Experimental Validation Results for G9P/CD158D Antibody (YA7099)
  • G9P/CD158D Antibody (YA7099) Product Image
  • Experimental Validation Results for G9P/CD158D Antibody (YA7099)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD158D Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for G9P/CD158D Antibody (YA7099)
  • G9P/CD158D Antibody (YA7099) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling CD158D Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • G9P/CD158D Antibody (YA7099) Product Image FC